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Sandbox Wabash 13
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==Distinguishing between the active site and a nearby dicarboxylic acid binding site in Fumarase== | ==Distinguishing between the active site and a nearby dicarboxylic acid binding site in Fumarase== | ||
| - | In early studies, two carboxylic acid binding sites, subsequently named A- and B-, were observed in the wild type crystal structure of fumarase C in ''E. Coli.'' The structures of these two sites was considerably different as site A contained atom of three of the four subunits of fumarase C whereas site B contained only atoms from a single subunit of the tetramer. While it was heavily believed that the carboxylic acid binding site A- was the location of the fumarase active site, it could not be verified without first mutating both active sites in order to determine the activity of the reactions <ref>PMID:9098893</references> | + | In early studies, two carboxylic acid binding sites, subsequently named A- and B-, were observed in the wild type crystal structure of fumarase C in ''E. Coli.'' The structures of these two sites was considerably different as site A contained atom of three of the four subunits of fumarase C whereas site B contained only atoms from a single subunit of the tetramer. While it was heavily believed that the carboxylic acid binding site A- was the location of the fumarase active site, it could not be verified without first mutating both active sites in order to determine the activity of the reactions <ref>PMID:9098893</references>. Prior data had suggested that a histidine residue was a critical base in the catalysis and therefore H188 (located in the A- site) and H129 (located in the B-site) were mutated to asparagines. |
== Function == | == Function == | ||
Revision as of 17:00, 28 February 2016
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