Sandbox Wabash 11 Fumarase

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(→The Active Site of Fumarase C)
(→The Active Site of Fumarase C)
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'''The Debate About Two Possible Locations of the Active Site'''
'''The Debate About Two Possible Locations of the Active Site'''
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Many studies of Fumarase have shown that there are two different binding sites for carboxylic acid. One of the two sites (Site A), is formed by the residues from three subunits and is located at the tungstate sight. The active site is very deep and contains His-188, Ser-98, Thr-100, Asn-141. Site A also utilizes hydrogen bonding between a water molecule and His-188 to increase stability of substrate. The second site (Site B), is formed from atoms in a single subunit and contains L-malate. Site B is close to the surface of the enzymes and is composed of Asn-131, Asp-132, His-129, and Arg-126. A study conducted by Todd Weaver, Mason Lees, and Leonard Banaszak confirmed that Site A was the actual active site by mutating the Histidine residues at each site into Arginine and observing which mutation most effected substrate binding.
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Many studies of Fumarase have shown that there are two different binding sites for carboxylic acid. One of the two sites (Site A), is formed by the residues from three subunits and is located at the tungstate sight. The active site is very deep and contains His-188, Ser-98, Thr-100, Asn-141. Site A also utilizes hydrogen bonding between a water molecule and His-188 to increase stability of substrate. The second site (Site B), is formed from atoms in a single subunit and contains L-malate. Site B is close to the surface of the enzymes and is composed of Asn-131, Asp-132, His-129, and Arg-126. A study conducted by Todd Weaver, Mason Lees, and Leonard Banaszak confirmed that Site A was the actual active site by mutating the <scene name='72/726380/Acitve_site_a_mutated/1'>Histidine residue (Site A displayed)</scene> at each site into Arginine and observing which mutation most effected substrate binding.

Revision as of 22:42, 28 February 2016

The Active Site of Fumarase C

Fumarase C

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References

  1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
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