1gjr

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[[Image:1gjr.jpg|left|200px]]
[[Image:1gjr.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1gjr |SIZE=350|CAPTION= <scene name='initialview01'>1gjr</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1gjr", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=FAD:Nap+Binding+Site+For+Chain+A'>FAD</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1gjr| PDB=1gjr | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjr OCA], [http://www.ebi.ac.uk/pdbsum/1gjr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gjr RCSB]</span>
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}}
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'''FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION'''
'''FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION'''
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Mechanism of coenzyme recognition and binding revealed by crystal structure analysis of ferredoxin-NADP+ reductase complexed with NADP+., Hermoso JA, Mayoral T, Faro M, Gomez-Moreno C, Sanz-Aparicio J, Medina M, J Mol Biol. 2002 Jun 21;319(5):1133-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12079352 12079352]
Mechanism of coenzyme recognition and binding revealed by crystal structure analysis of ferredoxin-NADP+ reductase complexed with NADP+., Hermoso JA, Mayoral T, Faro M, Gomez-Moreno C, Sanz-Aparicio J, Medina M, J Mol Biol. 2002 Jun 21;319(5):1133-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12079352 12079352]
[[Category: Anabaena sp.]]
[[Category: Anabaena sp.]]
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[[Category: Ferredoxin--NADP(+) reductase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Gomez-Moreno, C.]]
[[Category: Gomez-Moreno, C.]]
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[[Category: Medina, M.]]
[[Category: Medina, M.]]
[[Category: Sanz-Aparicio, J.]]
[[Category: Sanz-Aparicio, J.]]
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[[Category: fad]]
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[[Category: Fad]]
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[[Category: flavoprotein]]
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[[Category: Flavoprotein]]
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[[Category: fnr]]
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[[Category: Fnr]]
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[[Category: nadp]]
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[[Category: Nadp]]
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[[Category: nadp reductase]]
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[[Category: Nadp reductase]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:39:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:45:29 2008''
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Revision as of 14:39, 2 May 2008

Image:1gjr.jpg

Template:STRUCTURE 1gjr

FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION


Overview

The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyses the production of NADPH in photosynthesis. The three-dimensional structure of FNR presents two distinct domains, one for binding of the FAD prosthetic group and the other for NADP+ binding. In spite of extensive experiments and different crystallographic approaches, many aspects about how the NADP+ substrate binds to FNR and how the hydride ion is transferred from FAD to NADP+ remain unclear. The structure of an FNR:NADP+ complex from Anabaena has been determined by X-ray diffraction analysis of the cocrystallised units to 2.1 A resolution. Structural perturbation of FNR induced by complex formation produces a narrower cavity in which the 2'-phospho-AMP and pyrophosphate portions of the NADP+ are perfectly bound. In addition, the nicotinamide mononucleotide moiety is placed in a new pocket created near the FAD cofactor with the ribose being in a tight conformation. The crystal structure of this FNR:NADP+ complex obtained by cocrystallisation displays NADP+ in an unusual conformation and can be considered as an intermediate state in the process of coenzyme recognition and binding. Structural analysis and comparison with previously reported complexes allow us to postulate a mechanism which would permit efficient hydride transfer to occur. Besides, this structure gives new insights into the postulated formation of the ferredoxin:FNR:NADP+ ternary complex by prediction of new intermolecular interactions, which could only exist after FNR:NADP+ complex formation. Finally, structural comparison with the members of the broad FNR structural family also provides an explanation for the high specificity exhibited by FNR for NADP+/H versus NAD+/H.

About this Structure

1GJR is a Single protein structure of sequence from Anabaena sp.. Full crystallographic information is available from OCA.

Reference

Mechanism of coenzyme recognition and binding revealed by crystal structure analysis of ferredoxin-NADP+ reductase complexed with NADP+., Hermoso JA, Mayoral T, Faro M, Gomez-Moreno C, Sanz-Aparicio J, Medina M, J Mol Biol. 2002 Jun 21;319(5):1133-42. PMID:12079352 Page seeded by OCA on Fri May 2 17:39:40 2008

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