1gk8

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[[Image:1gk8.gif|left|200px]]
[[Image:1gk8.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1gk8 |SIZE=350|CAPTION= <scene name='initialview01'>1gk8</scene>, resolution 1.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1gk8", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=MGA:Active+Site+Residues+Coordinating+Mg'>MGA</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MME:N-METHYL+METHIONINE'>MME</scene>, <scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1gk8| PDB=1gk8 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gk8 OCA], [http://www.ebi.ac.uk/pdbsum/1gk8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gk8 RCSB]</span>
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}}
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'''RUBISCO FROM CHLAMYDOMONAS REINHARDTII'''
'''RUBISCO FROM CHLAMYDOMONAS REINHARDTII'''
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[[Category: Spreitzer, R J.]]
[[Category: Spreitzer, R J.]]
[[Category: Taylor, T C.]]
[[Category: Taylor, T C.]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: photosynthesis]]
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[[Category: Photosynthesis]]
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[[Category: rubisco]]
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[[Category: Rubisco]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:40:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:45:47 2008''
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Revision as of 14:40, 2 May 2008

Image:1gk8.gif

Template:STRUCTURE 1gk8

RUBISCO FROM CHLAMYDOMONAS REINHARDTII


Overview

The crystal structure of Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) from the unicellular green alga Chlamydomonas reinhardtii has been determined to 1.4 A resolution. Overall, the structure shows high similarity to the previously determined structures of L8S8 Rubisco enzymes. The largest difference is found in the loop between beta strands A and B of the small subunit (betaA-betaB loop), which is longer by six amino acid residues than the corresponding region in Rubisco from Spinacia. Mutations of residues in the betaA-betaB loop have been shown to affect holoenzyme stability and catalytic properties. The information contained in the Chlamydomonas structure enables a more reliable analysis of the effect of these mutations. No electron density was observed for the last 13 residues of the small subunit, which are assumed to be disordered in the crystal. Because of the high resolution of the data, some posttranslational modifications are unambiguously apparent in the structure. These include cysteine and N-terminal methylations and proline 4-hydroxylations.

About this Structure

1GK8 is a Protein complex structure of sequences from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii., Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I, J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:11641402 Page seeded by OCA on Fri May 2 17:40:50 2008

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