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1goi

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[[Image:1goi.gif|left|200px]]
[[Image:1goi.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1goi |SIZE=350|CAPTION= <scene name='initialview01'>1goi</scene>, resolution 1.45&Aring;
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The line below this paragraph, containing "STRUCTURE_1goi", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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{{STRUCTURE_1goi| PDB=1goi | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1goi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1goi OCA], [http://www.ebi.ac.uk/pdbsum/1goi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1goi RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE D140N MUTANT OF CHITINASE B FROM SERRATIA MARCESCENS AT 1.45 A RESOLUTION'''
'''CRYSTAL STRUCTURE OF THE D140N MUTANT OF CHITINASE B FROM SERRATIA MARCESCENS AT 1.45 A RESOLUTION'''
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[[Category: Kolstad, G.]]
[[Category: Kolstad, G.]]
[[Category: Synstad, B.]]
[[Category: Synstad, B.]]
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[[Category: chitin degradation]]
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[[Category: Chitin degradation]]
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[[Category: glycosidase]]
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[[Category: Glycosidase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:49:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:48:12 2008''
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Revision as of 14:49, 2 May 2008

Image:1goi.gif

Template:STRUCTURE 1goi

CRYSTAL STRUCTURE OF THE D140N MUTANT OF CHITINASE B FROM SERRATIA MARCESCENS AT 1.45 A RESOLUTION


Overview

The crystal structure of the inactive D140N mutant of Serratia marcescens was refined to 1.45 A resolution. The structure of the mutant was essentially identical to that of the wild type, with the exception of a rotation of Asp142 in the catalytic centre. In the mutant, this residue interacts with the catalytic acid (Glu144) and not with residue 140 as in the wild type. Thus, the 500-fold decrease in activity in the D140N mutant seems to be largely mediated by an effect on Asp142, confirming the crucial role of the latter residue in catalysis.

About this Structure

1GOI is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.

Reference

Structure of the D140N mutant of chitinase B from Serratia marcescens at 1.45 A resolution., Kolstad G, Synstad B, Eijsink VG, van Aalten DM, Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):377-9. Epub 2002, Jan 24. PMID:11807282 Page seeded by OCA on Fri May 2 17:49:33 2008

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