Gelsolin
From Proteopedia
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<StructureSection load='1h1v' size='350' side='right' caption='Human gelsolin G4-G6 (gold) complex with actin (grey), ATP (stick model) and Ca+2 ion (green) (PDB entry [[1h1v]])' scene=''> | <StructureSection load='1h1v' size='350' side='right' caption='Human gelsolin G4-G6 (gold) complex with actin (grey), ATP (stick model) and Ca+2 ion (green) (PDB entry [[1h1v]])' scene=''> | ||
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== Function == | == Function == | ||
| - | [[Gelsolin]] (GLS) is a protein regulator of actin assembly and disassembly<ref>PMID:10559185</ref>. | + | [[Gelsolin]] (GLS) is a protein regulator of actin assembly and disassembly<ref>PMID:10559185</ref>. Binding of Ca+2 ion to GLS induces conformational change which enables it to bind actin. |
== Structural highlights == | == Structural highlights == | ||
| - | + | GLS contains 6 homologous domains G1 to G6. The human GLS domains span residues: 15-135, 136-248, 249-367, 394-513, 514-619 and 620-734. The binding site of Ca+2 ion includes Glu, Asp and 2 carbonyl oxygens<ref>PMID:12460571</ref>. | |
| - | + | </StructureSection> | |
== 3D Structures of GLS == | == 3D Structures of GLS == | ||
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**[[2x1o]], [[2x1p]], [[2x1q]] – GLS nanobody - Llama | **[[2x1o]], [[2x1p]], [[2x1q]] – GLS nanobody - Llama | ||
| - | * GLS domains | + | * GLS domains G1-G3 |
**[[3ffk]] – hGLS +actin <br /> | **[[3ffk]] – hGLS +actin <br /> | ||
**[[1rgi]] - hoGLS +actin <br /> | **[[1rgi]] - hoGLS +actin <br /> | ||
| - | * GLS domains | + | * GLS domains G4-G6 |
| - | **[[1h1v]] - hGLS +actin<br /> | + | **[[1h1v]] - hGLS +actin + Ca + ATP<br /> |
**[[1nph]] - mGLS +Ca – mouse<br /> | **[[1nph]] - mGLS +Ca – mouse<br /> | ||
| - | * GLS domain | + | * GLS domain G1 |
**[[2ff3]] - hGLS +N-WASP+actin<br /> | **[[2ff3]] - hGLS +N-WASP+actin<br /> | ||
Revision as of 09:34, 6 March 2016
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3D Structures of GLS
Updated on 06-March-2016
References
- ↑ Sun HQ, Yamamoto M, Mejillano M, Yin HL. Gelsolin, a multifunctional actin regulatory protein. J Biol Chem. 1999 Nov 19;274(47):33179-82. PMID:10559185
- ↑ Choe H, Burtnick LD, Mejillano M, Yin HL, Robinson RC, Choe S. The calcium activation of gelsolin: insights from the 3A structure of the G4-G6/actin complex. J Mol Biol. 2002 Dec 6;324(4):691-702. PMID:12460571
