1grz
From Proteopedia
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'''A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME''' | '''A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GRZ OCA]. | |
==Reference== | ==Reference== | ||
A preorganized active site in the crystal structure of the Tetrahymena ribozyme., Golden BL, Gooding AR, Podell ER, Cech TR, Science. 1998 Oct 9;282(5387):259-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9841391 9841391] | A preorganized active site in the crystal structure of the Tetrahymena ribozyme., Golden BL, Gooding AR, Podell ER, Cech TR, Science. 1998 Oct 9;282(5387):259-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9841391 9841391] | ||
| - | [[Category: Protein complex]] | ||
| - | [[Category: Tetrahymena thermophila]] | ||
[[Category: Cech, T R.]] | [[Category: Cech, T R.]] | ||
[[Category: Golden, B L.]] | [[Category: Golden, B L.]] | ||
[[Category: Gooding, A R.]] | [[Category: Gooding, A R.]] | ||
[[Category: Podell, E R.]] | [[Category: Podell, E R.]] | ||
| - | [[Category: | + | [[Category: Ribozyme structure]] |
| - | [[Category: | + | [[Category: Rrna group i intron]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:56:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:56, 2 May 2008
A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME
Overview
Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5' splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5' splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme.
About this Structure
Full crystallographic information is available from OCA.
Reference
A preorganized active site in the crystal structure of the Tetrahymena ribozyme., Golden BL, Gooding AR, Podell ER, Cech TR, Science. 1998 Oct 9;282(5387):259-64. PMID:9841391 Page seeded by OCA on Fri May 2 17:56:18 2008
