This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5i4a
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA== | |
| + | <StructureSection load='5i4a' size='340' side='right' caption='[[5i4a]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5i4a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4A FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4a OCA], [http://pdbe.org/5i4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4a RCSB], [http://www.ebi.ac.uk/pdbsum/5i4a PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Eukaryotic Argonaute proteins induce gene silencing by small RNA-guided recognition and cleavage of mRNA targets. Although structural similarities between human and prokaryotic Argonautes are consistent with shared mechanistic properties, sequence and structure-based alignments suggested that Argonautes encoded within CRISPR-cas[clustered regularly interspaced short palindromic repeats (CRISPR)-associated] bacterial immunity operons have divergent activities. We show here that the CRISPR-associatedMarinitoga piezophilaArgonaute (MpAgo) protein cleaves single-stranded target sequences using 5'-hydroxylated guide RNAs rather than the 5'-phosphorylated guides used by all known Argonautes. The 2.0-A resolution crystal structure of an MpAgo-RNA complex reveals a guide strand binding site comprising residues that block 5' phosphate interactions. Using structure-based sequence alignment, we were able to identify other putative MpAgo-like proteins, all of which are encoded within CRISPR-casloci. Taken together, our data suggest the evolution of an Argonaute subclass with noncanonical specificity for a 5'-hydroxylated guide. | ||
| - | + | A bacterial Argonaute with noncanonical guide RNA specificity.,Kaya E, Doxzen KW, Knoll KR, Wilson RC, Strutt SC, Kranzusch PJ, Doudna JA Proc Natl Acad Sci U S A. 2016 Mar 30. pii: 201524385. PMID:27035975<ref>PMID:27035975</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5i4a" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | + | <references/> | |
| - | [[Category: Doxzen, K | + | __TOC__ |
| - | + | </StructureSection> | |
| + | [[Category: Doudna, J A]] | ||
| + | [[Category: Doxzen, K W]] | ||
[[Category: Kaya, E]] | [[Category: Kaya, E]] | ||
| - | [[Category: | + | [[Category: Knoll, K R]] |
| + | [[Category: Kranzusch, P J]] | ||
| + | [[Category: Strutt, S C]] | ||
| + | [[Category: Wilson, R C]] | ||
| + | [[Category: 5' oh]] | ||
| + | [[Category: Argonaute]] | ||
| + | [[Category: Guide rna]] | ||
| + | [[Category: Rna binding protein-rna complex]] | ||
Revision as of 19:40, 10 May 2016
X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA
| |||||||||||
