1gsp

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[[Image:1gsp.gif|left|200px]]
[[Image:1gsp.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1gsp |SIZE=350|CAPTION= <scene name='initialview01'>1gsp</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1gsp", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=BI1:Site'>BI1</scene>, <scene name='pdbsite=CAL:Site'>CAL</scene> and <scene name='pdbsite=CAT:Active+Site'>CAT</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SGP:GUANOSINE-2&#39;,3&#39;-CYCLOPHOSPHOROTHIOATE'>SGP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1gsp| PDB=1gsp | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsp OCA], [http://www.ebi.ac.uk/pdbsum/1gsp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gsp RCSB]</span>
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}}
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'''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY'''
'''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY'''
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Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546218 9546218]
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546218 9546218]
[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
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[[Category: Ribonuclease T(1)]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Wyns, L.]]
[[Category: Wyns, L.]]
[[Category: Zegers, I.]]
[[Category: Zegers, I.]]
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[[Category: endoribonuclease]]
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[[Category: Endoribonuclease]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:57:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:50:28 2008''
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Revision as of 14:57, 2 May 2008

Image:1gsp.gif

Template:STRUCTURE 1gsp

RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY


Overview

Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.

About this Structure

1GSP is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218 Page seeded by OCA on Fri May 2 17:57:43 2008

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