1gw9

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[[Image:1gw9.gif|left|200px]]
[[Image:1gw9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1gw9 |SIZE=350|CAPTION= <scene name='initialview01'>1gw9</scene>, resolution 1.55&Aring;
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The line below this paragraph, containing "STRUCTURE_1gw9", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CA1:Lxc+Binding+Site+For+Chain+A'>CA1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=LXC:BETA-L-XYLOPYRANOSE'>LXC</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1gw9| PDB=1gw9 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gw9 OCA], [http://www.ebi.ac.uk/pdbsum/1gw9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gw9 RCSB]</span>
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}}
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'''TRI-IODIDE DERIVATIVE OF XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS'''
'''TRI-IODIDE DERIVATIVE OF XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS'''
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[[Category: Bricogne, G.]]
[[Category: Bricogne, G.]]
[[Category: Evans, G.]]
[[Category: Evans, G.]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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[[Category: magnesium]]
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[[Category: Magnesium]]
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[[Category: pentose shunt]]
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[[Category: Pentose shunt]]
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[[Category: xylose metabolism]]
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[[Category: Xylose metabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:05:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:52:33 2008''
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Revision as of 15:05, 2 May 2008

Image:1gw9.gif

Template:STRUCTURE 1gw9

TRI-IODIDE DERIVATIVE OF XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS


Overview

A series of experiments performed at Cu Kalpha wavelength on in-house X-ray equipment are presented which investigate two possibilities for enhancing the experimental phasing signal by means of (i) triiodide/iodide soaks using KI/I(2) and (ii) combinations of counter-ions introduced using the short cryosoak method. Triiodide-derivative crystal structures for five test proteins have been refined and reveal that iodine can bind as polyiodide and single iodide ions through hydrophobic and hydrogen-bonding interactions both at the molecular surface and in intramolecular and intermolecular cavities. In three cases, the structures could be automatically determined with autoSHARP using in-house SAD and SIRAS data. The investigation of combinatorial counter-ion replacement using multiple salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals that, for the case of hen egg-white lysozyme, significant improvement in phasing signal is obtained by the combined use of salts compared with SIRAS methods using native and single short-soak derivative data sets.

About this Structure

1GW9 is a Single protein structure of sequence from Streptomyces rubiginosus. Full crystallographic information is available from OCA.

Reference

Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300 Page seeded by OCA on Fri May 2 18:05:22 2008

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