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1gwd
From Proteopedia
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'''TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME''' | '''TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME''' | ||
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[[Category: Bricogne, G.]] | [[Category: Bricogne, G.]] | ||
[[Category: Evans, G.]] | [[Category: Evans, G.]] | ||
| - | [[Category: | + | [[Category: Aller]] |
| - | [[Category: | + | [[Category: Bacteriolytic enzyme]] |
| - | [[Category: | + | [[Category: Glycosidase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Signal]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:05:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:05, 2 May 2008
TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME
Overview
A series of experiments performed at Cu Kalpha wavelength on in-house X-ray equipment are presented which investigate two possibilities for enhancing the experimental phasing signal by means of (i) triiodide/iodide soaks using KI/I(2) and (ii) combinations of counter-ions introduced using the short cryosoak method. Triiodide-derivative crystal structures for five test proteins have been refined and reveal that iodine can bind as polyiodide and single iodide ions through hydrophobic and hydrogen-bonding interactions both at the molecular surface and in intramolecular and intermolecular cavities. In three cases, the structures could be automatically determined with autoSHARP using in-house SAD and SIRAS data. The investigation of combinatorial counter-ion replacement using multiple salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals that, for the case of hen egg-white lysozyme, significant improvement in phasing signal is obtained by the combined use of salts compared with SIRAS methods using native and single short-soak derivative data sets.
About this Structure
1GWD is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300 Page seeded by OCA on Fri May 2 18:05:41 2008
