1h34
From Proteopedia
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'''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR''' | '''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR''' | ||
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[[Category: Girmann, B.]] | [[Category: Girmann, B.]] | ||
[[Category: Sheldrick, G M.]] | [[Category: Sheldrick, G M.]] | ||
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| - | [[Category: | + | [[Category: Serine protease inhibitor]] |
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Revision as of 15:22, 2 May 2008
CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR
Overview
SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.
About this Structure
1H34 is a Single protein structure of sequence from Phaseolus lunatus. Full crystallographic information is available from OCA.
Reference
In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:12554963 Page seeded by OCA on Fri May 2 18:22:06 2008
