1h4h

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[[Image:1h4h.gif|left|200px]]
[[Image:1h4h.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1h4h |SIZE=350|CAPTION= <scene name='initialview01'>1h4h</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1h4h", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=BC3:Xys+Binding+Site+For+Residue+D1211'>BC3</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1h4h| PDB=1h4h | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4h OCA], [http://www.ebi.ac.uk/pdbsum/1h4h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h4h RCSB]</span>
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}}
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'''OLIGOSACCHARIDE-BINDING TO FAMILY 11 XYLANASES: BOTH COVALENT INTERMEDIATE AND MUTANT-PRODUCT COMPLEXES DISPLAY 2,5B CONFORMATIONS AT THE ACTIVE-CENTRE'''
'''OLIGOSACCHARIDE-BINDING TO FAMILY 11 XYLANASES: BOTH COVALENT INTERMEDIATE AND MUTANT-PRODUCT COMPLEXES DISPLAY 2,5B CONFORMATIONS AT THE ACTIVE-CENTRE'''
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[[Category: Schulein, M.]]
[[Category: Schulein, M.]]
[[Category: Wilson, K S.]]
[[Category: Wilson, K S.]]
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[[Category: boat conformation]]
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[[Category: Boat conformation]]
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[[Category: intermediate]]
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[[Category: Intermediate]]
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[[Category: mutant]]
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[[Category: Mutant]]
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[[Category: oligosaccharide]]
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[[Category: Oligosaccharide]]
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[[Category: transition-state]]
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[[Category: Transition-state]]
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[[Category: xylanase]]
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[[Category: Xylanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:25:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:57:30 2008''
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Revision as of 15:25, 2 May 2008

Image:1h4h.gif

Template:STRUCTURE 1h4h

OLIGOSACCHARIDE-BINDING TO FAMILY 11 XYLANASES: BOTH COVALENT INTERMEDIATE AND MUTANT-PRODUCT COMPLEXES DISPLAY 2,5B CONFORMATIONS AT THE ACTIVE-CENTRE


Overview

The glycoside hydrolase sequence-based classification reveals two families of enzymes which hydrolyse the beta-1,4-linked backbone of xylan, xylanases, termed families GH-10 and GH-11. Family GH-11 xylanases are intriguing in that catalysis is performed via a covalent intermediate adopting an unusual (2,5)B (boat) conformation, a conformation which also fulfils the stereochemical constraints of the oxocarbenium ion-like transition state. Here, the 1.9 A structure of a nucleophile, E94A, mutant of the Xyn11 from Bacillus agaradhaerens in complex with xylotriose is presented. Intriguingly, this complex also adopts the (2,5)B conformation in the -1 subsite, with the vacant space provided by the Glu-->Ala mutation allowing the sugar to adopt the alpha-configuration at C1. The structure of the covalent 2-deoxy-2-fluoroxylobiosyl-enzyme intermediate has been extended to atomic (1.1 A) resolution.

About this Structure

1H4H is a Single protein structure of sequence from Bacillus agaradhaerens. Full crystallographic information is available from OCA.

Reference

Oligosaccharide binding to family 11 xylanases: both covalent intermediate and mutant product complexes display (2,5)B conformations at the active centre., Sabini E, Wilson KS, Danielsen S, Schulein M, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1344-7. Epub 2001, Aug 23. PMID:11526340 Page seeded by OCA on Fri May 2 18:25:15 2008

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OCA

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