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Introduction

Rickets is a disease caused by a vitamin D deficiency. Vitamin D can be obtained from ultra violet radiation and from various food sources. Cytochrome P450 enzymes are involved in the first step to regulate and process vitamin D in the human body.

Overall Structure

- Asymmetric dimer
- Consists of α-helices, β-sheets (mostly on one side of the molecule) with a heme buried inside the protein
- Two molecules of 2-hydroxypropyl-β-cyclodextrin are found near the dimer interface

Binding Interactions

-catalyzes initial step for converting vitamin D into 25-hydroxyvitamin D -mutation causes rickets-25-hydroxylase deficiency -has closed conformation, substrate access channel mostly covered -secosteroid binding, extended active site

Additional Features

This molecule has a heme which is bound to iron, which, combined with its structural conformation, allows for hydroxylation with the attached substrate. This molecule carries out important functions and is not species or sex specific.

Quiz Question 1

(merely an example of what this section might look like)

rom can you identify the green, red, and blue parts of the molecule?

See Also

• Cytochrome P450
• Drug Metabolism by CYP450 Enzymes
• 3w0y
• 3w0c

Credits

Introduction - Sami Kriksceonaitis

Overall Structure - Kati Johnson

Drug Binding Site - Isabel Hand1

Additional Features - Elizabeth Humble

Quiz Question 1 - Matthew Tiller

References

1. ↑ Strushkevich N, Usanov SA, Plotnikov AN, Jones G, Park HW. Structural analysis of CYP2R1 in complex with vitamin D3. J Mol Biol. 2008 Jun 27;380(1):95-106. Epub 2008 Apr 8. PMID:18511070 doi:10.1016/j.jmb.2008.03.065