1h9c
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1h9c.gif|left|200px]] | [[Image:1h9c.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1h9c", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1h9c| PDB=1h9c | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.''' | '''NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.''' | ||
| Line 24: | Line 21: | ||
NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11352587 11352587] | NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11352587 11352587] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ab, E.]] | [[Category: Ab, E.]] | ||
| Line 33: | Line 29: | ||
[[Category: Scheek, R M.]] | [[Category: Scheek, R M.]] | ||
[[Category: Schuurman-Wolters, G.]] | [[Category: Schuurman-Wolters, G.]] | ||
| - | [[Category: | + | [[Category: Enzyme iib-chitobiose]] |
| - | [[Category: | + | [[Category: Iib-cellobiose]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Phosphotransferase system]] |
| - | [[Category: | + | [[Category: Sugar transport]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:35:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:35, 2 May 2008
NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.
Overview
The determination by NMR of the solution structure of the phosphorylated enzyme IIB (P-IIB(Chb)) of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli is presented. Most of the backbone and side-chain resonances were assigned using a variety of mostly heteronuclear NMR experiments. The remaining resonances were assigned with the help of the structure calculations.NOE-derived distance restraints were used in distance geometry calculations followed by molecular dynamics and simulated annealing protocols. In addition, combinations of ambiguous restraints were used to resolve ambiguities in the NOE assignments. By combining sets of ambiguous and unambiguous restraints into new ambiguous restraints, an error function was constructed that was less sensitive to information loss caused by assignment uncertainties. The final set of structures had a pairwise rmsd of 0.59 A and 1.16 A for the heavy atoms of the backbone and side-chains, respectively.Comparing the P-IIB(Chb) solution structure with the previously determined NMR and X-ray structures of the wild-type and the Cys10Ser mutant shows that significant differences between the structures are limited to the active-site region. The phosphoryl group at the active-site cysteine residue is surrounded by a loop formed by residues 10 through 16. NOE and chemical shift data suggest that the phosphoryl group makes hydrogen bonds with the backbone amide protons of residues 12 and 15. The binding mode of the phosphoryl group is very similar to that of the protein tyrosine phosphatases. The differences observed are in accordance with the presumption that IIB(Chb) has to be more resistant to hydrolysis than the protein tyrosine phosphatases. We propose a proton relay network by which a transfer occurs between the cysteine SH proton and the solvent via the hydroxyl group of Thr16.
About this Structure
1H9C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:11352587 Page seeded by OCA on Fri May 2 18:35:57 2008
