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1he8

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[[Image:1he8.gif|left|200px]]
[[Image:1he8.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1he8 |SIZE=350|CAPTION= <scene name='initialview01'>1he8</scene>, resolution 3.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1he8", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene> and <scene name='pdbsite=AC2:Gnp+Binding+Site+For+Chain+C'>AC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_3-kinase Phosphatidylinositol 3-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.137 2.7.1.137] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1he8| PDB=1he8 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1he8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1he8 OCA], [http://www.ebi.ac.uk/pdbsum/1he8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1he8 RCSB]</span>
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}}
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'''RAS G12V-PI 3-KINASE GAMMA COMPLEX'''
'''RAS G12V-PI 3-KINASE GAMMA COMPLEX'''
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[[Category: Walker, E H.]]
[[Category: Walker, E H.]]
[[Category: Williams, R L.]]
[[Category: Williams, R L.]]
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[[Category: gmppnp]]
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[[Category: Gmppnp]]
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[[Category: gtp]]
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[[Category: Gtp]]
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[[Category: h-ras g12v]]
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[[Category: H-ras g12v]]
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[[Category: heat domain]]
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[[Category: Heat domain]]
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[[Category: oncogene protein]]
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[[Category: Oncogene protein]]
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[[Category: phosphatidylinositol 3-kinase]]
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[[Category: Phosphatidylinositol 3-kinase]]
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[[Category: phosphoinositide 3-kinase gamma - h-ras g12v complex]]
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[[Category: Phosphoinositide 3-kinase gamma - h-ras g12v complex]]
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[[Category: pi 3-k]]
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[[Category: Pi 3-k]]
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[[Category: pi 3-kinase]]
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[[Category: Pi 3-kinase]]
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[[Category: pi3k]]
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[[Category: Pi3k]]
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[[Category: ras effector]]
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[[Category: Ras effector]]
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[[Category: ras-binding domain]]
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[[Category: Ras-binding domain]]
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[[Category: second messenger generation]]
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[[Category: Second messenger generation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:45:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:03:20 2008''
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Revision as of 15:45, 2 May 2008

Image:1he8.gif

Template:STRUCTURE 1he8

RAS G12V-PI 3-KINASE GAMMA COMPLEX


Overview

Ras activation of phosphoinositide 3-kinase (PI3K) is important for survival of transformed cells. We find that PI3Kgamma is strongly and directly activated by H-Ras G12V in vivo or by GTPgammaS-loaded H-Ras in vitro. We have determined a crystal structure of a PI3Kgamma/Ras.GMPPNP complex. A critical loop in the Ras binding domain positions Ras so that it uses its switch I and switch II regions to bind PI3Kgamma. Mutagenesis shows that interactions with both regions are essential for binding PI3Kgamma. Ras also forms a direct contact with the PI3Kgamma catalytic domain. These unique Ras/PI3Kgamma interactions are likely to be shared by PI3Kalpha. The complex with Ras shows a change in the PI3K conformation that may represent an allosteric component of Ras activation.

About this Structure

1HE8 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure and functional analysis of Ras binding to its effector phosphoinositide 3-kinase gamma., Pacold ME, Suire S, Perisic O, Lara-Gonzalez S, Davis CT, Walker EH, Hawkins PT, Stephens L, Eccleston JF, Williams RL, Cell. 2000 Dec 8;103(6):931-43. PMID:11136978 Page seeded by OCA on Fri May 2 18:45:27 2008

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