1hji

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[[Image:1hji.gif|left|200px]]
[[Image:1hji.gif|left|200px]]
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{{Structure
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|PDB= 1hji |SIZE=350|CAPTION= <scene name='initialview01'>1hji</scene>
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The line below this paragraph, containing "STRUCTURE_1hji", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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{{STRUCTURE_1hji| PDB=1hji | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hji OCA], [http://www.ebi.ac.uk/pdbsum/1hji PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hji RCSB]</span>
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}}
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'''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX'''
'''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX'''
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[[Category: Schaerpf, M.]]
[[Category: Schaerpf, M.]]
[[Category: Sticht, H.]]
[[Category: Sticht, H.]]
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[[Category: bacteriophage hk022]]
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[[Category: Bacteriophage hk022]]
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[[Category: peptide-rna-complex]]
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[[Category: Peptide-rna-complex]]
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[[Category: peptide-rna-recognition]]
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[[Category: Peptide-rna-recognition]]
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[[Category: protein/rna]]
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[[Category: Protein/rna]]
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[[Category: termination]]
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[[Category: Termination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:54:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:10 2008''
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Revision as of 15:54, 2 May 2008

Image:1hji.gif

Template:STRUCTURE 1hji

BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX


Overview

Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.

About this Structure

1HJI is a Protein complex structure of sequences from Enterobacteria phage hk022. Full crystallographic information is available from OCA.

Reference

The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination., Faber C, Scharpf M, Becker T, Sticht H, Rosch P, J Biol Chem. 2001 Aug 24;276(34):32064-70. Epub 2001 May 16. PMID:11356847 Page seeded by OCA on Fri May 2 18:54:38 2008

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