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4qmd

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Revision as of 09:32, 24 April 2019

Crystal structure of human envoplakin plakin repeat domain

Structural highlights

4qmd is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	EVPL (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EVPL_HUMAN] Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.

Publication Abstract from PubMed

Plakin proteins form critical connections between cell junctions and the cytoskeleton; their disruption within epithelial and cardiac muscle cells cause skin-blistering diseases and cardiomyopathies. Envoplakin has a single plakin repeat domain (PRD) which recognizes intermediate filaments through an unresolved mechanism. Herein we report the crystal structure of envoplakin's complete PRD fold, revealing binding determinants within its electropositive binding groove. Four of its five internal repeats recognize negatively charged patches within vimentin via five basic determinants that are identified by nuclear magnetic resonance spectroscopy. Mutations of the Lys1901 or Arg1914 binding determinants delocalize heterodimeric envoplakin from intracellular vimentin and keratin filaments in cultured cells. Recognition of vimentin is abolished when its residues Asp112 or Asp119 are mutated. The latter slot intermediate filament rods into basic PRD domain grooves through electrosteric complementarity in a widely applicable mechanism. Together this reveals how plakin family members form dynamic linkages with cytoskeletal frameworks.

Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin.,Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M Nat Commun. 2016 Mar 3;7:10827. doi: 10.1038/ncomms10827. PMID:26935805^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M. Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin. Nat Commun. 2016 Mar 3;7:10827. doi: 10.1038/ncomms10827. PMID:26935805 doi:http://dx.doi.org/10.1038/ncomms10827

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4qmd"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human envoplakin plakin repeat domain==
-<StructureSection load='4qmd' size='340' side='right' caption='[[4qmd]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='4qmd' size='340' side='right'caption='[[4qmd]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4qmd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QMD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4qmd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QMD FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmd OCA], [http://pdbe.org/4qmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qmd RCSB], [http://www.ebi.ac.uk/pdbsum/4qmd PDBsum]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EVPL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qmd OCA], [http://pdbe.org/4qmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qmd RCSB], [http://www.ebi.ac.uk/pdbsum/4qmd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qmd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Al-Jassar, C]]
 [[Category: Chidgey, M]]

4qmd

From Proteopedia

Revision as of 09:32, 24 April 2019

Crystal structure of human envoplakin plakin repeat domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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