1hk6
From Proteopedia
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'''RAL BINDING DOMAIN FROM SEC5''' | '''RAL BINDING DOMAIN FROM SEC5''' | ||
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[[Category: Nietlispach, D.]] | [[Category: Nietlispach, D.]] | ||
[[Category: Owen, D.]] | [[Category: Owen, D.]] | ||
| - | [[Category: | + | [[Category: Immunoglobulin fold]] |
| - | [[Category: | + | [[Category: Ipt ral sec5 exocyst,exocytosis,protein transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:56:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:56, 2 May 2008
RAL BINDING DOMAIN FROM SEC5
Overview
The exocyst complex is involved in the final stages of exocytosis, when vesicles are targeted to the plasma membrane and dock. The regulation of exocytosis is vital for a number of processes, for example, cell polarity, embryogenesis, and neuronal growth formation. Regulation of the exocyst complex in mammals was recently shown to be dependent upon binding of the small G protein, Ral, to Sec5, a central component of the exocyst. This interaction is thought to be necessary for anchoring the exocyst to secretory vesicles. We have determined the structure of the Ral-binding domain of Sec5 and shown that it adopts a fold that has not been observed in a G protein effector before. This fold belongs to the immunoglobulin superfamily in a subclass known as IPT domains. We have mapped the Ral binding site on this domain and found that it overlaps with protein-protein interaction sites on other IPT domains but that it is completely different from the G protein-geranyl-geranyl interaction face of the Ig-like domain of the Rho guanine nucleotide dissociation inhibitor. This mapping, along with available site-directed mutagenesis data, allows us to predict how Ral and Sec5 may interact.
About this Structure
1HK6 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of the GTPase-binding domain of Sec5 and elucidation of its Ral binding site., Mott HR, Nietlispach D, Hopkins LJ, Mirey G, Camonis JH, Owen D, J Biol Chem. 2003 May 9;278(19):17053-9. Epub 2003 Mar 6. PMID:12624092 Page seeded by OCA on Fri May 2 18:56:07 2008
