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1hl3
From Proteopedia
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'''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE''' | '''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE''' | ||
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[[Category: Pesce, A.]] | [[Category: Pesce, A.]] | ||
[[Category: Spano , S.]] | [[Category: Spano , S.]] | ||
| - | [[Category: | + | [[Category: Acyl-coa]] |
| - | [[Category: | + | [[Category: Acyltransferase]] |
| - | [[Category: | + | [[Category: Brefeldin some]] |
| - | [[Category: | + | [[Category: Golgi membrane]] |
| - | [[Category: | + | [[Category: Nad]] |
| - | [[Category: | + | [[Category: Transcription co-repression]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:58:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:58, 2 May 2008
CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE
Overview
C-terminal-binding protein/brefeldin A-ADP ribosylated substrate (CtBP/BARS) plays key roles in development and oncogenesis as a transcription co-repressor, and in intracellular traffic as a promoter of Golgi membrane fission. Co-repressor activity is regulated by NAD(H) binding to CtBP/BARS, while membrane fission is associated with its acyl-CoA-dependent acyltransferase activity. Here, we report the crystal structures of rat CtBP/BARS in a binary complex with NAD(H), and in a ternary complex with a PIDLSKK peptide mimicking the consensus motif (PXDLS) recognized in CtBP/BARS cellular partners. The structural data show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps the recognition site for DNA-binding proteins and histone deacetylases to an N-terminal region of the protein. The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes.
About this Structure
1HL3 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:12805226 Page seeded by OCA on Fri May 2 18:58:12 2008
