1hkz

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[[Image:1hkz.gif|left|200px]]
[[Image:1hkz.gif|left|200px]]
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{{Structure
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|PDB= 1hkz |SIZE=350|CAPTION= <scene name='initialview01'>1hkz</scene>, resolution 2.49&Aring;
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The line below this paragraph, containing "STRUCTURE_1hkz", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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{{STRUCTURE_1hkz| PDB=1hkz | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkz OCA], [http://www.ebi.ac.uk/pdbsum/1hkz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hkz RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF BACILLUS SUBTILIS COTA AFTER 8H SOAKING WITH ABTS'''
'''CRYSTAL STRUCTURE OF BACILLUS SUBTILIS COTA AFTER 8H SOAKING WITH ABTS'''
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[[Category: Marcal, D.]]
[[Category: Marcal, D.]]
[[Category: Martins, L O.]]
[[Category: Martins, L O.]]
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[[Category: endospore coat]]
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[[Category: Endospore coat]]
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[[Category: laccase]]
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[[Category: Laccase]]
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[[Category: oxidase,copper-binding]]
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[[Category: Oxidase,copper-binding]]
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[[Category: reaction cycle,sporulation]]
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[[Category: Reaction cycle,sporulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:57:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:07:02 2008''
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Revision as of 15:57, 2 May 2008

Image:1hkz.gif

Template:STRUCTURE 1hkz

CRYSTAL STRUCTURE OF BACILLUS SUBTILIS COTA AFTER 8H SOAKING WITH ABTS


Overview

The CotA laccase from the endospore coat of Bacillus subtilis has been crystallized in the presence of the non-catalytic co-oxidant 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the structure was determined using synchrotron radiation. The binding site for this adduct is well defined and indicates how ABTS, in conjunction with laccases, could act as an oxidative mediator toward non-phenolic moieties. In addition, a dioxygen moiety is clearly defined within the solvent channel oriented toward one of the T3 copper atoms in the trinuclear center.

About this Structure

1HKZ is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis., Enguita FJ, Marcal D, Martins LO, Grenha R, Henriques AO, Lindley PF, Carrondo MA, J Biol Chem. 2004 May 28;279(22):23472-6. Epub 2004 Feb 4. PMID:14764581 Page seeded by OCA on Fri May 2 18:57:54 2008

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