This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1hmh
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1hmh.jpg|left|200px]] | [[Image:1hmh.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1hmh", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1hmh| PDB=1hmh | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME''' | '''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA]. | |
==Reference== | ==Reference== | ||
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422] | Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Flaherty, K M.]] | [[Category: Flaherty, K M.]] | ||
[[Category: McKay, D B.]] | [[Category: McKay, D B.]] | ||
[[Category: Pley, H W.]] | [[Category: Pley, H W.]] | ||
| - | [[Category: | + | [[Category: Dna-rna hammerhead ribozyme]] |
| - | [[Category: | + | [[Category: Loop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:00:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:00, 2 May 2008
THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Overview
The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.
About this Structure
Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422 Page seeded by OCA on Fri May 2 19:00:55 2008
