1hpm

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[[Image:1hpm.gif|left|200px]]
[[Image:1hpm.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1hpm |SIZE=350|CAPTION= <scene name='initialview01'>1hpm</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1hpm", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1hpm| PDB=1hpm | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hpm OCA], [http://www.ebi.ac.uk/pdbsum/1hpm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hpm RCSB]</span>
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}}
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'''HOW POTASSIUM AFFECTS THE ACTIVITY OF THE MOLECULAR CHAPERONE HSC70. II. POTASSIUM BINDS SPECIFICALLY IN THE ATPASE ACTIVE SITE'''
'''HOW POTASSIUM AFFECTS THE ACTIVITY OF THE MOLECULAR CHAPERONE HSC70. II. POTASSIUM BINDS SPECIFICALLY IN THE ATPASE ACTIVE SITE'''
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[[Category: Mckay, D B.]]
[[Category: Mckay, D B.]]
[[Category: Wilbanks, S M.]]
[[Category: Wilbanks, S M.]]
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[[Category: hydrolase (acting on acid anhydrides)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:05:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:08:46 2008''
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Revision as of 16:05, 2 May 2008

Image:1hpm.gif

Template:STRUCTURE 1hpm

HOW POTASSIUM AFFECTS THE ACTIVITY OF THE MOLECULAR CHAPERONE HSC70. II. POTASSIUM BINDS SPECIFICALLY IN THE ATPASE ACTIVE SITE


Overview

Crystallographic anomalous scattering from potassium at 1.7 A resolution reveals two monovalent ions that interact with MgADP and P(i) in the nucleotide binding cleft of wild-type recombinant bovine Hsc70 ATPase fragment. K+ at site 1 interacts with oxygens of the beta-phosphate of ADP, whereas K+ at site 2 interacts with an oxygen of P(i). Both K+ ions also interact with specific H2O molecules in the first hydration shell of the octahedrally coordinated Mg2+ ion and with specific protein ligands. In crystals that have Na+ present, K+ is replaced by a Na+ ion at site 1 and by a Na(+)-H2O pair at site 2. The K+ ions are positioned where they could stabilize binding of a beta,gamma-bidentate MgATP complex with Hsc70, as well as a transition state during ATP hydrolysis, suggesting that monovalent ions act as specific metal cofactors in the ATPase reaction of Hsc70.

About this Structure

1HPM is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

How potassium affects the activity of the molecular chaperone Hsc70. II. Potassium binds specifically in the ATPase active site., Wilbanks SM, McKay DB, J Biol Chem. 1995 Feb 3;270(5):2251-7. PMID:7836458 Page seeded by OCA on Fri May 2 19:05:54 2008

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