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1hqy
From Proteopedia
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'''Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU''' | '''Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU''' | ||
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[[Category: Song, J J.]] | [[Category: Song, J J.]] | ||
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
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| - | [[Category: | + | [[Category: Peptidase-atpase complex]] |
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Revision as of 16:08, 2 May 2008
Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU
Overview
BACKGROUND: The bacterial heat shock locus ATPase HslU is an AAA(+) protein that has structures known in many nucleotide-free and -bound states. Nucleotide is required for the formation of the biologically active HslU hexameric assembly. The hexameric HslU ATPase binds the dodecameric HslV peptidase and forms an ATP-dependent HslVU protease. RESULTS: We have characterized four distinct HslU conformational states, going sequentially from open to closed: the empty, SO(4), ATP, and ADP states. The nucleotide binds at a cleft formed by an alpha/beta domain and an alpha-helical domain in HslU. The four HslU states differ by a rotation of the alpha-helical domain. This classification leads to a correction of nucleotide identity in one structure and reveals the ATP hydrolysis-dependent structural changes in the HslVU complex, including a ring rotation and a conformational change of the HslU C terminus. This leads to an amended protein unfolding-coupled translocation mechanism. CONCLUSIONS: The observed nucleotide-dependent conformational changes in HslU and their governing principles provide a framework for the mechanistic understanding of other AAA(+) proteins.
About this Structure
1HQY is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Nucleotide-dependent conformational changes in a protease-associated ATPase HsIU., Wang J, Song JJ, Seong IS, Franklin MC, Kamtekar S, Eom SH, Chung CH, Structure. 2001 Nov;9(11):1107-16. PMID:11709174 Page seeded by OCA on Fri May 2 19:08:41 2008
