1hqz
From Proteopedia
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'''Cofilin homology domain of a yeast actin-binding protein ABP1P''' | '''Cofilin homology domain of a yeast actin-binding protein ABP1P''' | ||
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Strokopytov, B V.]] | [[Category: Strokopytov, B V.]] | ||
| - | [[Category: | + | [[Category: Actin binding]] |
| - | [[Category: | + | [[Category: Cofilin homology domain]] |
| - | [[Category: | + | [[Category: New york structural genomix research consortium]] |
| - | [[Category: | + | [[Category: Nysgxrc]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
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Revision as of 16:08, 2 May 2008
Cofilin homology domain of a yeast actin-binding protein ABP1P
Overview
A modified molecular-replacement method is described that makes use of six-dimensional searches and the phased translation function, providing a systematic examination of all possible search-model orientations in an experimental electron-density map. As an example, the structure solution of the cofilin-homology domain of the Saccharomyces cerevisiae actin-binding protein 1 (ABP1) is presented in detail. Additional examples are presented in which these tools have significantly aided structure solutions in a variety of contexts. These results suggest that this approach might be of widespread utility for challenging structures involving weak phase information, complex asymmetric units and search models with weak structural homology. Furthermore, this approach supports an exhaustive molecular-replacement strategy in cases where an appropriate search model cannot readily be identified on the basis of sequence homology. The fully automated web-based implementation of this phased translation function is described.
About this Structure
1HQZ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches., Strokopytov BV, Fedorov A, Mahoney NM, Kessels M, Drubin DG, Almo SC, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):285-93. Epub 2005, Feb 24. PMID:15735338 Page seeded by OCA on Fri May 2 19:08:45 2008
Categories: Saccharomyces cerevisiae | Single protein | Almo, S C. | Burley, S K. | Drubin, D G. | Fedorov, A A. | Mahoney, N. | NYSGXRC, New York Structural GenomiX Research Consortium. | Strokopytov, B V. | Actin binding | Cofilin homology domain | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomic
