This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5hiw
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Sorangium cellulosum So Ce56 cytochrome P450 260B1== | |
| + | <StructureSection load='5hiw' size='340' side='right' caption='[[5hiw]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hiw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HIW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hiw OCA], [http://pdbe.org/5hiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hiw RCSB], [http://www.ebi.ac.uk/pdbsum/5hiw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hiw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Myxobacterial CYP260B1 from Sorangium cellulosum was heterologously expressed in Escherichia coli and purified. The in vitro conversion of a small focused substrate library comprised of Delta4 C21-steroids and steroidal drugs using surrogate bovine redox partners shows that CYP260B1 is a novel steroid hydroxylase. CYP260B1 performs the regio- and stereoselective hydroxylation of the glucocorticoid cortodoxone (RSS) to produce 6beta-OH-RSS. The substrate-free crystal structure of CYP260B1 (PDB 5HIW) was resolved. Docking of the tested ligands into the crystal structure suggested that the C17 hydroxy moiety and the presence of either a keto or a hydroxy group at C11 determine the selectivity of hydroxylation. | ||
| - | + | Structure-function analysis for the hydroxylation of Delta4 C21-steroids by the myxobacterial CYP260B1.,Salamanca-Pinzon SG, Khatri Y, Carius Y, Keller L, Muller R, Lancaster CR, Bernhardt R FEBS Lett. 2016 Jun;590(12):1838-51. doi: 10.1002/1873-3468.12217. Epub 2016 Jun , 3. PMID:27177597<ref>PMID:27177597</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5hiw" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bernhardt, R]] | [[Category: Bernhardt, R]] | ||
| - | [[Category: Salamanca-Pinzon, S | + | [[Category: Carius, Y]] |
| + | [[Category: Khatri, Y]] | ||
| + | [[Category: Lancaster, C R.D]] | ||
| + | [[Category: Salamanca-Pinzon, S G]] | ||
| + | [[Category: Cyp]] | ||
| + | [[Category: Cytochrome]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: P450]] | ||
| + | [[Category: Sorangium]] | ||
| + | [[Category: Steroid]] | ||
Revision as of 03:59, 4 August 2016
Sorangium cellulosum So Ce56 cytochrome P450 260B1
| |||||||||||
Categories: Bernhardt, R | Carius, Y | Khatri, Y | Lancaster, C R.D | Salamanca-Pinzon, S G | Cyp | Cytochrome | Oxidoreductase | P450 | Sorangium | Steroid
