1hrs
From Proteopedia
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'''A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN''' | '''A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN''' | ||
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[[Category: Precigoux, G.]] | [[Category: Precigoux, G.]] | ||
[[Category: Yariv, J.]] | [[Category: Yariv, J.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:10:14 2008'' | |
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Revision as of 16:10, 2 May 2008
A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN
Overview
Ferritin, the iron-storage protein, binds porphyrins, metalloporphyrins and the fluorescent dyes ANS (8-anilino-1-naphthalenesulfonic acid) and TNS (2-p-toluidinyl-6-naphthalenesulfonic acid), similarly to apo-myoglobin. Octahedral crystals of horse-spleen apo-ferritin (HSF; 174 amino acids) complexes prepared by the addition of haem, hematoporphyrin or Sn-protoporphyrin IX to a solution of apo-ferritin crystallize in space group F432 with cell parameter a = 184.0 A. X-ray crystallographic analysis of single crystals prepared from a mixture containing haem or Sn-protoporphyrin IX shows that the haem-binding sites in these crystals are occupied by protoporphyrin IX, which is free of metal, rather than by the original metalloporphyrin. The present paper describes the structure of horse-spleen apo-ferritin cocrystallized with Sn-protoporphyrin IX. The 6797 reflections up to 2.6 A resolution used in the refinement were obtained from a data set recorded on a Nicolet/Xentronics area detector with Cu Kalpha radiation from a Rigaku RU 200 rotating anode. The final structure comprises 1613 non-H atoms, two Cd atoms and 170 solvent molecules. Four residues are described as disordered. The root-mean-square deviations from ideal bond lengths and angles are 0.013 A and 2.88 degrees, respectively. Protoporphyrins are observed in special positions on the twofold axes of the ferritin molecule with a stoichiometry of 0.4 per subunit.
About this Structure
1HRS is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
A crystallographic study of haem binding to ferritin., Precigoux G, Yariv J, Gallois B, Courseille C, d'Estaintot BL, Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):739-43. PMID:15299370 Page seeded by OCA on Fri May 2 19:10:14 2008
