1ht9
From Proteopedia
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[[Image:1ht9.gif|left|200px]] | [[Image:1ht9.gif|left|200px]] | ||
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'''DOMAIN SWAPPING EF-HANDS''' | '''DOMAIN SWAPPING EF-HANDS''' | ||
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[[Category: Linse, S.]] | [[Category: Linse, S.]] | ||
[[Category: Svensson, A L.]] | [[Category: Svensson, A L.]] | ||
| - | [[Category: | + | [[Category: Calbindin d9k]] |
| - | [[Category: | + | [[Category: Calcium binding]] |
| - | [[Category: | + | [[Category: Domain swapping]] |
| - | [[Category: | + | [[Category: Ef-hand]] |
| - | [[Category: | + | [[Category: Folding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:12:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:12, 2 May 2008
DOMAIN SWAPPING EF-HANDS
Overview
The structure of calbindin D(9k) with two substitutions was determined by X-ray crystallography at 1.8-A resolution. Unlike wild-type calbindin D(9k), which is a monomeric protein with two EF-hands, the structure of the mutated calbindin D(9k) reveals an intertwined dimer. In the dimer, two EF-hands of the monomers have exchanged places, and thus a 3D domain-swapped dimer has been formed. EF-hand I of molecule A is packed toward EF-hand II of molecule B and vice versa. The formation of a hydrophobic cluster, in a region linking the EF-hands, promotes the conversion of monomers to 3D domain-swapped dimers. We propose a mechanism by which domain swapping takes place via the apo form of calbindin D(9k). Once formed, the calbindin D(9k) dimers are remarkably stable, as with even larger misfolded aggregates like amyloids. Thus calbindin D(9k) dimers cannot be converted to monomers by dilution. However, heating can be used for conversion, indicating high energy barriers separating monomers from dimers.
About this Structure
1HT9 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
An extended hydrophobic core induces EF-hand swapping., Hakansson M, Svensson A, Fast J, Linse S, Protein Sci. 2001 May;10(5):927-33. PMID:11316872 Page seeded by OCA on Fri May 2 19:12:29 2008
