This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1hwp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1hwp.gif|left|200px]]
[[Image:1hwp.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1hwp |SIZE=350|CAPTION= <scene name='initialview01'>1hwp</scene>, resolution 3.1&Aring;
+
The line below this paragraph, containing "STRUCTURE_1hwp", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PT1:PTEROIC+ACID'>PT1</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1hwp| PDB=1hwp | SCENE= }}
-
|RELATEDENTRY=[[1hwn|1HWN]], [[1hwo|1HWO]], [[1hwm|1HWM]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwp OCA], [http://www.ebi.ac.uk/pdbsum/1hwp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hwp RCSB]</span>
+
-
}}
+
'''EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM'''
'''EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM'''
Line 19: Line 16:
==About this Structure==
==About this Structure==
-
1HWP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWP OCA].
+
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWP OCA].
==Reference==
==Reference==
2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11288182 11288182]
2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11288182 11288182]
-
[[Category: Protein complex]]
+
[[Category: RRNA N-glycosylase]]
-
[[Category: Sambucus ebulus]]
+
-
[[Category: rRNA N-glycosylase]]
+
[[Category: Day, P J.]]
[[Category: Day, P J.]]
[[Category: Ernst, S R.]]
[[Category: Ernst, S R.]]
Line 31: Line 26:
[[Category: Pascal, J M.]]
[[Category: Pascal, J M.]]
[[Category: Robertus, J D.]]
[[Category: Robertus, J D.]]
-
[[Category: inhibitor]]
+
[[Category: Inhibitor]]
-
[[Category: ribosome-inactivating protein]]
+
[[Category: Ribosome-inactivating protein]]
-
[[Category: ricin-like]]
+
[[Category: Ricin-like]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:12 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:11:21 2008''
+

Revision as of 16:18, 2 May 2008

Image:1hwp.gif

Template:STRUCTURE 1hwp

EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM


Overview

Ebulin l is a type-II ribosome-inactivating protein (RIP) isolated from the leaves of Sambucus ebulus L. As with other type-II RIP, ebulin is a disulfide-linked heterodimer composed of a toxic A chain and a galactoside-specific lectin B chain. A normal level of ribosome-inactivating N-glycosidase activity, characteristic of the A chain of type-II RIP, has been demonstrated for ebulin l. However, ebulin is considered a nontoxic type-II RIP due to a reduced cytotoxicity on whole cells and animals as compared with other toxic type-II RIP like ricin. The molecular cloning, amino acid sequence, and the crystal structure of ebulin l are presented and compared with ricin. Ebulin l is shown to bind an A-chain substrate analogue, pteroic acid, in the same manner as ricin. The galactoside-binding ability of ebulin l is demonstrated crystallographically with a complex of the B chain with galactose and with lactose. The negligible cytotoxicity of ebulin l is apparently due to a reduced affinity for galactosides. An altered mode of galactoside binding in the 2gamma subdomain of the lectin B chain primarily causes the reduced affinity.

About this Structure

Full crystallographic information is available from OCA.

Reference

2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:11288182 Page seeded by OCA on Fri May 2 19:18:12 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hwp"
Personal tools