1hwq
From Proteopedia
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[[Image:1hwq.gif|left|200px]] | [[Image:1hwq.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1hwq| PDB=1hwq | SCENE= }} | |
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'''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP''' | '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA]. | |
==Reference== | ==Reference== | ||
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11350168 11350168] | A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11350168 11350168] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Dieckmann, T.]] | [[Category: Dieckmann, T.]] | ||
[[Category: Flinders, J C.]] | [[Category: Flinders, J C.]] | ||
| - | [[Category: | + | [[Category: A+c base pair]] |
| - | [[Category: | + | [[Category: Protonated adenine]] |
| - | [[Category: | + | [[Category: Stem-loop]] |
| - | [[Category: | + | [[Category: Tandem ga]] |
| - | [[Category: | + | [[Category: Vs ribozyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:18, 2 May 2008
SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP
Overview
The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.
About this Structure
Full crystallographic information is available from OCA.
Reference
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168 Page seeded by OCA on Fri May 2 19:18:14 2008
