1hxd
From Proteopedia
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[[Image:1hxd.gif|left|200px]] | [[Image:1hxd.gif|left|200px]] | ||
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'''CRYSTAL STRUCTURE OF E. COLI BIOTIN REPRESSOR WITH BOUND BIOTIN''' | '''CRYSTAL STRUCTURE OF E. COLI BIOTIN REPRESSOR WITH BOUND BIOTIN''' | ||
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==Reference== | ==Reference== | ||
Corepressor-induced organization and assembly of the biotin repressor: a model for allosteric activation of a transcriptional regulator., Weaver LH, Kwon K, Beckett D, Matthews BW, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6045-50. Epub 2001 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11353844 11353844] | Corepressor-induced organization and assembly of the biotin repressor: a model for allosteric activation of a transcriptional regulator., Weaver LH, Kwon K, Beckett D, Matthews BW, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6045-50. Epub 2001 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11353844 11353844] | ||
| - | [[Category: Biotin--[acetyl-CoA-carboxylase] ligase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Ruparelia, S.]] | [[Category: Ruparelia, S.]] | ||
[[Category: Streaker, E D.]] | [[Category: Streaker, E D.]] | ||
| - | [[Category: | + | [[Category: Biotin]] |
| - | [[Category: | + | [[Category: Dna-binding]] |
| - | [[Category: | + | [[Category: Ligase]] |
| - | [[Category: | + | [[Category: Repressor]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:19:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:19, 2 May 2008
CRYSTAL STRUCTURE OF E. COLI BIOTIN REPRESSOR WITH BOUND BIOTIN
Overview
The Escherichia coli biotin repressor binds to the biotin operator to repress transcription of the biotin biosynthetic operon. In this work, a structure determined by x-ray crystallography of a complex of the repressor bound to biotin, which also functions as an activator of DNA binding by the biotin repressor (BirA), is described. In contrast to the monomeric aporepressor, the complex is dimeric with an interface composed in part of an extended beta-sheet. Model building, coupled with biochemical data, suggests that this is the dimeric form of BirA that binds DNA. Segments of three surface loops that are disordered in the aporepressor structure are located in the interface region of the dimer and exhibit greater order than was observed in the aporepressor structure. The results suggest that the corepressor of BirA causes a disorder-to-order transition that is a prerequisite to repressor dimerization and DNA binding.
About this Structure
1HXD is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Corepressor-induced organization and assembly of the biotin repressor: a model for allosteric activation of a transcriptional regulator., Weaver LH, Kwon K, Beckett D, Matthews BW, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6045-50. Epub 2001 May 15. PMID:11353844 Page seeded by OCA on Fri May 2 19:19:32 2008
