1hz1

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[[Image:1hz1.gif|left|200px]]
[[Image:1hz1.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1hz1 |SIZE=350|CAPTION= <scene name='initialview01'>1hz1</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1hz1", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=2GP:GUANOSINE-2&#39;-MONOPHOSPHATE'>2GP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1hz1| PDB=1hz1 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hz1 OCA], [http://www.ebi.ac.uk/pdbsum/1hz1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hz1 RCSB]</span>
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}}
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'''RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH MG2+'''
'''RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH MG2+'''
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The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution., Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R, Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11453993 11453993]
The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution., Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R, Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11453993 11453993]
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Ribonuclease T(1)]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Langhorst, U.]]
[[Category: Langhorst, U.]]
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[[Category: Swarte, J De.]]
[[Category: Swarte, J De.]]
[[Category: Vos, S De.]]
[[Category: Vos, S De.]]
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[[Category: metal binding]]
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[[Category: Metal binding]]
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[[Category: ribonuclease]]
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[[Category: Ribonuclease]]
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[[Category: stability]]
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[[Category: Stability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:22:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:12:15 2008''
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Revision as of 16:22, 2 May 2008

Image:1hz1.gif

Template:STRUCTURE 1hz1

RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH MG2+


Overview

In the crystalline state, ribonuclease T1 binds calcium ions at different lattice-dependent positions. In solution, its conformational stability is also remarkably increased in the presence of divalent metal ions. Combining urea unfolding studies and X-ray crystallography, we compared the presence of several metal ions at specific sites in the protein to their contribution to the overall stabilizing effect in solution. We constructed thermodynamic cycles involving particular metal ions and specific carboxylate functions. The resulting coupling energies indicate that some (but not all) metal ions found at lattice contacts in crystal structures may indeed significantly contribute to stability enhancement in the presence of metal ions in solution.

About this Structure

1HZ1 is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.

Reference

The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution., Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R, Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:11453993 Page seeded by OCA on Fri May 2 19:22:55 2008

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