5jlg
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The X-ray structure of the adduct formed in the reaction between bovine pancreatic ribonuclease and compound I, a piano-stool organometallic Ru(II) arene compound containing an O,S-chelating ligand== |
| + | <StructureSection load='5jlg' size='340' side='right' caption='[[5jlg]], [[Resolution|resolution]] 1.79Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5jlg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JLG FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6L6:METHYL+(2Z)-3-HYDROXY-3-(3-HYDROXYPHENYL)PROP-2-ENE(DITHIOATE)'>6L6</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=RU:RUTHENIUM+ION'>RU</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jlg OCA], [http://pdbe.org/5jlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jlg RCSB], [http://www.ebi.ac.uk/pdbsum/5jlg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jlg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN]] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A new pseudo-octahedral pi-arene ruthenium(ii) piano-stool compound, containing an O,S-bidentate ligand (compound 1) and showing significant cytotoxic activity in vitro, was synthesized and characterized. In solution stability and interaction with the model protein bovine pancreatic ribonuclease (RNase A) were investigated by using UV-Vis absorption spectroscopy. Its crystal structure and that of the adduct formed upon reaction with RNase A were obtained by X-ray crystallography. The comparison between the structure of purified compound 1 and that of the fragment bound to RNase A reveals an unusual mode of protein binding that includes ligand exchange and alteration of coordination sphere geometry. | ||
| - | + | Unusual mode of protein binding by a cytotoxic pi-arene ruthenium(ii) piano-stool compound containing an O,S-chelating ligand.,Hildebrandt J, Gorls H, Hafner N, Ferraro G, Durst M, Runnebaum IB, Weigand W, Merlino A Dalton Trans. 2016 Aug 2;45(31):12283-7. doi: 10.1039/c6dt02380k. PMID:27427335<ref>PMID:27427335</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5jlg" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Ferraro, G]] | [[Category: Ferraro, G]] | ||
[[Category: Merlino, A]] | [[Category: Merlino, A]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Protein-ru compound adduct]] | ||
| + | [[Category: Ruthenated protein]] | ||
Revision as of 04:05, 4 August 2016
The X-ray structure of the adduct formed in the reaction between bovine pancreatic ribonuclease and compound I, a piano-stool organometallic Ru(II) arene compound containing an O,S-chelating ligand
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