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5ejo

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Revision as of 18:42, 12 July 2016

Crystal structure of the winged helix domain in Chromatin assembly factor 1 subunit p90

Structural highlights

5ejo is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RLF2_YEAST] Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p90 may facilitate the efficient and timely assembly of histones into telomeric chromatin.

Publication Abstract from PubMed

Chromatin assembly factor 1 (CAF-1) is a histone H3-H4 chaperone that deposits newly synthesized histone (H3-H4)2 tetramers during replication-coupled nucleosome assembly. However, how CAF-1 functions in this process is not yet well understood. Here, we report the crystal structure of C terminus of Cac1 (Cac1C), a subunit of yeast CAF-1, and the function of this domain in stabilizing CAF-1 at replication forks. We show that Cac1C forms a winged helix domain (WHD) and binds DNA in a sequence-independent manner. Mutations in Cac1C that abolish DNA binding result in defects in transcriptional silencing and increased sensitivity to DNA damaging agents, and these defects are exacerbated when combined with Cac1 mutations deficient in PCNA binding. Similar phenotypes are observed for corresponding mutations in mouse CAF-1. These results reveal a mechanism conserved in eukaryotic cells whereby the ability of CAF-1 to bind DNA is important for its association with the DNA replication forks and subsequent nucleosome assembly.

A DNA binding winged helix domain in CAF-1 functions with PCNA to stabilize CAF-1 at replication forks.,Zhang K, Gao Y, Li J, Burgess R, Han J, Liang H, Zhang Z, Liu Y Nucleic Acids Res. 2016 Feb 22. pii: gkw106. PMID:26908650^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang K, Gao Y, Li J, Burgess R, Han J, Liang H, Zhang Z, Liu Y. A DNA binding winged helix domain in CAF-1 functions with PCNA to stabilize CAF-1 at replication forks. Nucleic Acids Res. 2016 Feb 22. pii: gkw106. PMID:26908650 doi:http://dx.doi.org/10.1093/nar/gkw106

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ejo"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5ejo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EJO FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ejo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejo OCA], [http://pdbe.org/5ejo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejo RCSB], [http://www.ebi.ac.uk/pdbsum/5ejo PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ejo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejo OCA], [http://pdbe.org/5ejo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejo RCSB], [http://www.ebi.ac.uk/pdbsum/5ejo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejo ProSAT]</span></td></tr>
 </table>
 == Function ==

5ejo

From Proteopedia

Revision as of 18:42, 12 July 2016

Crystal structure of the winged helix domain in Chromatin assembly factor 1 subunit p90

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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