1i6h

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[[Image:1i6h.jpg|left|200px]]
[[Image:1i6h.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1i6h |SIZE=350|CAPTION= <scene name='initialview01'>1i6h</scene>, resolution 3.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1i6h", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1i6h| PDB=1i6h | SCENE= }}
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|RELATEDENTRY=[[1i3q|1I3Q]], [[1i50|1I50]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i6h OCA], [http://www.ebi.ac.uk/pdbsum/1i6h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i6h RCSB]</span>
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}}
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'''RNA POLYMERASE II ELONGATION COMPLEX'''
'''RNA POLYMERASE II ELONGATION COMPLEX'''
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[[Category: Gnatt, A L.]]
[[Category: Gnatt, A L.]]
[[Category: Kornberg, R D.]]
[[Category: Kornberg, R D.]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: molecular machine]]
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[[Category: Molecular machine]]
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[[Category: mrna]]
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[[Category: Mrna]]
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[[Category: multiprotein complex]]
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[[Category: Multiprotein complex]]
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[[Category: transcription]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:37:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:15:15 2008''
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Revision as of 16:37, 2 May 2008

Image:1i6h.jpg

Template:STRUCTURE 1i6h

RNA POLYMERASE II ELONGATION COMPLEX


Overview

The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 A resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 3' end of the RNA in the nucleotide addition site. The 3' end is positioned above a pore, through which nucleotides may enter and through which RNA may be extruded during back-tracking. The 5'-most residue of the RNA is close to the point of entry to an exit groove. Changes in protein structure between the transcribing complex and free enzyme include closure of a clamp over the DNA and RNA and ordering of a series of "switches" at the base of the clamp to create a binding site complementary to the DNA-RNA hybrid. Protein-nucleic acid contacts help explain DNA and RNA strand separation, the specificity of RNA synthesis, "abortive cycling" during transcription initiation, and RNA and DNA translocation during transcription elongation.

About this Structure

1I6H is a Protein complex structure of sequences from Saccharomyces cerevisiae. The following page contains interesting information on the relation of 1I6H with [RNA Polymerase]. Full crystallographic information is available from OCA.

Reference

Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution., Gnatt AL, Cramer P, Fu J, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1876-82. Epub 2001 Apr 19. PMID:11313499 Page seeded by OCA on Fri May 2 19:37:44 2008

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OCA

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