1i6y
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1i6y.jpg|left|200px]] | [[Image:1i6y.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1i6y", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_1i6y| PDB=1i6y | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''NMR ENSEMBLE OF ION-SELECTIVE LIGAND A1 FOR PLATELET INTEGRIN ALPHAIIB-BETA3''' | '''NMR ENSEMBLE OF ION-SELECTIVE LIGAND A1 FOR PLATELET INTEGRIN ALPHAIIB-BETA3''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I6Y OCA]. | |
==Reference== | ==Reference== | ||
Selection and structure of ion-selective ligands for platelet integrin alpha IIb(beta) 3., Smith JW, Le Calvez H, Parra-Gessert L, Preece NE, Jia X, Assa-Munt N, J Biol Chem. 2002 Mar 22;277(12):10298-305. Epub 2001 Dec 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11748219 11748219] | Selection and structure of ion-selective ligands for platelet integrin alpha IIb(beta) 3., Smith JW, Le Calvez H, Parra-Gessert L, Preece NE, Jia X, Assa-Munt N, J Biol Chem. 2002 Mar 22;277(12):10298-305. Epub 2001 Dec 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11748219 11748219] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Assa-Munt, N.]] | [[Category: Assa-Munt, N.]] | ||
[[Category: Calvez, H Le.]] | [[Category: Calvez, H Le.]] | ||
| Line 30: | Line 26: | ||
[[Category: Preece, N E.]] | [[Category: Preece, N E.]] | ||
[[Category: Smith, J W.]] | [[Category: Smith, J W.]] | ||
| - | [[Category: | + | [[Category: Integrin]] |
| - | [[Category: | + | [[Category: Rgd]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:38:47 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:38, 2 May 2008
NMR ENSEMBLE OF ION-SELECTIVE LIGAND A1 FOR PLATELET INTEGRIN ALPHAIIB-BETA3
Overview
Integrins contain a number of divalent cation binding sites that control ligand binding affinity. Ions such as Ca(2+) and Mg(2+) bind to distinct sites on integrin and can have opposing effects on ligand binding. These effects are presumably brought about by alterations of the shape of the ligand binding pocket. To gain insight into the nature of these structural differences, we probed the integrin ligand binding site with an RGD-based library of unparalleled complexity. A cysteine-constrained phage library containing six random amino acids and the RGD motif present in seven different registers was used to select for ligands that exhibit ion-selective binding to integrin alpha(IIb)beta(3). The library was used to select for peptides that bind to the integrin alpha(IIb)beta(3) preferentially in Ca(2+) versus Mg(2+). Peptides were identified which bound selectively in each ion. The Ca(2+)-selective peptides had a range of sequences, with the only obvious consensus involving a motif that had four cysteine residues bonded in a 1,4:2,3 arrangement. Interestingly though, the Mg(2+)-selective peptides exhibited a well defined consensus motif containing Cys-X-aromatic-L/G-R-G-D-hydrophobic-R-R/K-Cys. As a first step toward understanding the structural basis for this selectivity, solution NMR structures were obtained for representatives of both sets of peptides. All peptides formed turns, with the RGD motif at the apex. The Mg(2+)-selected peptides contained a unique basic patch that protrudes from the base of the turn.
About this Structure
Full crystallographic information is available from OCA.
Reference
Selection and structure of ion-selective ligands for platelet integrin alpha IIb(beta) 3., Smith JW, Le Calvez H, Parra-Gessert L, Preece NE, Jia X, Assa-Munt N, J Biol Chem. 2002 Mar 22;277(12):10298-305. Epub 2001 Dec 17. PMID:11748219 Page seeded by OCA on Fri May 2 19:38:47 2008
