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1i74
From Proteopedia
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[[Image:1i74.gif|left|200px]] | [[Image:1i74.gif|left|200px]] | ||
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'''STREPTOCOCCUS MUTANS INORGANIC PYROPHOSPHATASE''' | '''STREPTOCOCCUS MUTANS INORGANIC PYROPHOSPHATASE''' | ||
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[[Category: Merckel, M C.]] | [[Category: Merckel, M C.]] | ||
[[Category: Salminen, A.]] | [[Category: Salminen, A.]] | ||
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Revision as of 16:39, 2 May 2008
STREPTOCOCCUS MUTANS INORGANIC PYROPHOSPHATASE
Overview
BACKGROUND: Streptococcus mutans pyrophosphatase (Sm-PPase) is a member of a relatively uncommon but widely dispersed sequence family (family II) of inorganic pyrophosphatases. A structure will answer two main questions: is it structurally similar to the family I PPases, and is the mechanism similar? RESULTS: The first family II PPase structure, that of homodimeric Sm-PPase complexed with metal and sulfate ions, has been solved by X-ray crystallography at 2.2 A resolution. The tertiary fold of Sm-PPase consists of a 189 residue alpha/beta N-terminal domain and a 114 residue mixed beta sheet C-terminal domain and bears no resemblance to family I PPase, even though the arrangement of active site ligands and the residues that bind them shows significant similarity. The preference for Mn2+ over Mg2+ in family II PPases is explained by the histidine ligands and bidentate carboxylate coordination. The active site is located at the domain interface. The C-terminal domain is hinged to the N-terminal domain and exists in both closed and open conformations. CONCLUSIONS: The active site similiarities, including a water coordinated to two metal ions, suggest that the family II PPase mechanism is "analogous" (not "homologous") to that of family I PPases. This is a remarkable example of convergent evolution. The large change in C-terminal conformation suggests that domain closure might be the mechanism by which Sm-PPase achieves specificity for pyrophosphate over other polyphosphates.
About this Structure
1I74 is a Single protein structure of sequence from Streptococcus mutans. Full crystallographic information is available from OCA.
Reference
Crystal structure of Streptococcus mutans pyrophosphatase: a new fold for an old mechanism., Merckel MC, Fabrichniy IP, Salminen A, Kalkkinen N, Baykov AA, Lahti R, Goldman A, Structure. 2001 Apr 4;9(4):289-97. PMID:11525166 Page seeded by OCA on Fri May 2 19:39:07 2008
