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4c0y

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Revision as of 22:18, 15 November 2017

Cryo-EM reconstruction of empty enterovirus 71 in complex with a neutralizing antibody E18

Structural highlights

4c0y is a 5 chain structure with sequence from Human enterovirus 71 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	4c0u, 4c10
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[A9X4C2_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

Publication Abstract from PubMed

Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 degrees C and 37 degrees C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles.

Neutralizing antibodies can initiate genome release from human enterovirus 71.,Plevka P, Lim PY, Perera R, Cardosa J, Suksatu A, Kuhn RJ, Rossmann MG Proc Natl Acad Sci U S A. 2014 Jan 27. PMID:24469789^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Plevka P, Lim PY, Perera R, Cardosa J, Suksatu A, Kuhn RJ, Rossmann MG. Neutralizing antibodies can initiate genome release from human enterovirus 71. Proc Natl Acad Sci U S A. 2014 Jan 27. PMID:24469789 doi:http://dx.doi.org/10.1073/pnas.1320624111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4c0y"

@@ Line 3: / Line 3: @@
 <StructureSection load='4c0y' size='340' side='right' caption='[[4c0y]], [[Resolution|resolution]] 16.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4c0y]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4c0y]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0Y FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c0u|4c0u]], [[4c10|4c10]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0y OCA], [http://pdbe.org/4c0y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c0y RCSB], [http://www.ebi.ac.uk/pdbsum/4c0y PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0y OCA], [http://pdbe.org/4c0y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c0y RCSB], [http://www.ebi.ac.uk/pdbsum/4c0y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0y ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Ev-71]]
+[[Category: Human enterovirus 71]]
 [[Category: Mus musculus]]
 [[Category: Cardosa, J]]

4c0y

From Proteopedia

Revision as of 22:18, 15 November 2017

Cryo-EM reconstruction of empty enterovirus 71 in complex with a neutralizing antibody E18

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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