1idp

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[[Image:1idp.gif|left|200px]]
[[Image:1idp.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1idp |SIZE=350|CAPTION= <scene name='initialview01'>1idp</scene>, resolution 1.45&Aring;
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The line below this paragraph, containing "STRUCTURE_1idp", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] </span>
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{{STRUCTURE_1idp| PDB=1idp | SCENE= }}
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|RELATEDENTRY=[[3std|3std]], [[4std|4std]], [[5std|5std]], [[6std|6std]], [[7std|7std]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idp OCA], [http://www.ebi.ac.uk/pdbsum/1idp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1idp RCSB]</span>
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'''Crystal structure of scytalone dehydratase F162A mutant in the unligated state'''
'''Crystal structure of scytalone dehydratase F162A mutant in the unligated state'''
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[[Category: Nakasako, M.]]
[[Category: Nakasako, M.]]
[[Category: Yamaguchi, I.]]
[[Category: Yamaguchi, I.]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: melanine biosynthesis]]
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[[Category: Melanine biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:53:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:18:26 2008''
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Revision as of 16:53, 2 May 2008

Image:1idp.gif

Template:STRUCTURE 1idp

Crystal structure of scytalone dehydratase F162A mutant in the unligated state


Overview

Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K.

About this Structure

1IDP is a Single protein structure of sequence from Magnaporthe grisea. Full crystallographic information is available from OCA.

Reference

Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:11752795 Page seeded by OCA on Fri May 2 19:53:07 2008

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