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Nitrate reductase

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{{STRUCTURE_2jim| PDB=2jim | SIZE=400| SCENE= |right|CAPTION=Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo (green) and molybdepterin guanine nucleotide (stick model), [[2jim]] }}
 
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<StructureSection load='2jiq' size='350' side='right' caption='Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo (green), NO3 and molybdepterin guanine nucleotide (stick model) (PDB entry [[2jiq]])' scene=''>
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'''Nitrate reductase''' (NR) catalyzes the reduction of NO<sub>3</sub> to NO<sub>2</sub> using NADPH<ref>PMID:13152110</ref>. NR active site contains Mo atom. Four types of NR are known: one eukaryotic assimilatory NR and 3 bacterial NRs – cytoplasmic assimilatory (NAC), membrane-bound respiratory (NAR) and periplasmic dissimilatory (NAP). The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
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== Function ==
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'''Nitrate reductase''' (NR) catalyzes the reduction of NO<sub>3</sub> to NO<sub>2</sub> using NADPH<ref>PMID:13152110</ref>. NR active site contains Mo atom. Four types of NR are known:<br />
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'''eukaryotic assimilatory NR'''<br />
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'''bacterial cytoplasmic assimilatory''' (NAC)<br />
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'''bacterial membrane-bound respiratory''' (NAR)<br />
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'''bacterial periplasmic dissimilatory''' (NAP).<br />
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The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
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== Structural highlights ==
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NAP active site includes a six-coordinated Mo atom which coordinates to 3 amino acids and 2 MGD molecules. The planery NO<sub>3</sub> molecule is guided to the active site by a funnel leading to the Mo atom<ref>PMID:18327621</ref>.
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</StructureSection>
==3D structures of nitrate reductase==
==3D structures of nitrate reductase==

Revision as of 09:17, 15 May 2016

Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo (green), NO3 and molybdepterin guanine nucleotide (stick model) (PDB entry 2jiq)

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3D structures of nitrate reductase

Updated on 15-May-2016

References

  1. ↑ NICHOLAS DJ, NASON A. Molybdenum and nitrate reductase. II. Molybdenum as a constituent of nitrate reductase. J Biol Chem. 1954 Mar;207(1):353-60. PMID:13152110
  2. ↑ Najmudin S, Gonzalez PJ, Trincao J, Coelho C, Mukhopadhyay A, Cerqueira NM, Romao CC, Moura I, Moura JJ, Brondino CD, Romao MJ. Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum. J Biol Inorg Chem. 2008 Mar 8;. PMID:18327621 doi:10.1007/s00775-008-0359-6

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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