1iim

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[[Image:1iim.jpg|left|200px]]
[[Image:1iim.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1iim |SIZE=350|CAPTION= <scene name='initialview01'>1iim</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1iim", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=TTP:THYMIDINE-5&#39;-TRIPHOSPHATE'>TTP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-1-phosphate_thymidylyltransferase Glucose-1-phosphate thymidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.24 2.7.7.24] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1iim| PDB=1iim | SCENE= }}
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|RELATEDENTRY=[[1iin|1IIN]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iim OCA], [http://www.ebi.ac.uk/pdbsum/1iim PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1iim RCSB]</span>
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}}
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'''thymidylyltransferase complexed with TTP'''
'''thymidylyltransferase complexed with TTP'''
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[[Category: Rajashankar, K R.]]
[[Category: Rajashankar, K R.]]
[[Category: Thorson, J S.]]
[[Category: Thorson, J S.]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:02:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:20:18 2008''
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Revision as of 17:02, 2 May 2008

Image:1iim.jpg

Template:STRUCTURE 1iim

thymidylyltransferase complexed with TTP


Overview

Metabolite glycosylation is affected by three classes of enzymes: nucleotidylyltransferases, which activate sugars as nucleotide diphospho-derivatives, intermediate sugar-modifying enzymes and glycosyltransferases, which transfer the final derivatized activated sugars to aglycon substrates. One of the first crystal structures of an enzyme responsible for the first step in this cascade, alpha-D-glucopyranosyl phosphate thymidylyltransferase (Ep) from Salmonella, in complex with product (UDP-Glc) and substrate (dTTP) is reported at 2.0 A and 2.1 A resolution, respectively. These structures, in conjunction with the kinetic characterization of Ep, clarify the catalytic mechanism of this important enzyme class. Structure-based engineering of Ep produced modified enzymes capable of utilizing 'unnatural' sugar phosphates not accepted by wild type Ep. The demonstrated ability to alter nucleotidylyltransferase specificity by design is an integral component of in vitro glycosylation systems developed for the production of diverse glycorandomized libraries.

About this Structure

1IIM is a Single protein structure of sequence from Salmonella enterica. Full crystallographic information is available from OCA.

Reference

Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomization., Barton WA, Lesniak J, Biggins JB, Jeffrey PD, Jiang J, Rajashankar KR, Thorson JS, Nikolov DB, Nat Struct Biol. 2001 Jun;8(6):545-51. PMID:11373625 Page seeded by OCA on Fri May 2 20:02:43 2008

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OCA

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