1ilo
From Proteopedia
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[[Image:1ilo.gif|left|200px]] | [[Image:1ilo.gif|left|200px]] | ||
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'''NMR structure of a thioredoxin, MtH895, from the archeon Methanobacterium thermoautotrophicum strain delta H.''' | '''NMR structure of a thioredoxin, MtH895, from the archeon Methanobacterium thermoautotrophicum strain delta H.''' | ||
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Identification of a novel archaebacterial thioredoxin: determination of function through structure., Bhattacharyya S, Habibi-Nazhad B, Amegbey G, Slupsky CM, Yee A, Arrowsmith C, Wishart DS, Biochemistry. 2002 Apr 16;41(15):4760-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11939770 11939770] | Identification of a novel archaebacterial thioredoxin: determination of function through structure., Bhattacharyya S, Habibi-Nazhad B, Amegbey G, Slupsky CM, Yee A, Arrowsmith C, Wishart DS, Biochemistry. 2002 Apr 16;41(15):4760-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11939770 11939770] | ||
[[Category: Methanothermobacter thermautotrophicus]] | [[Category: Methanothermobacter thermautotrophicus]] | ||
| - | [[Category: Phosphoadenylyl-sulfate reductase (thioredoxin)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bhattacharyya, S.]] | [[Category: Bhattacharyya, S.]] | ||
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[[Category: Sykes, B D.]] | [[Category: Sykes, B D.]] | ||
[[Category: Wishart, D S.]] | [[Category: Wishart, D S.]] | ||
| - | [[Category: | + | [[Category: Beta-alpha-beta-alpha-beta-beta-alpha motif]] |
| - | [[Category: | + | [[Category: Nesg]] |
| - | [[Category: | + | [[Category: Northeast structural genomics consortium]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:07:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:07, 2 May 2008
NMR structure of a thioredoxin, MtH895, from the archeon Methanobacterium thermoautotrophicum strain delta H.
Overview
As part of a high-throughput, structural proteomic project we have used NMR spectroscopy to determine the solution structure and ascertain the function of a previously unknown, conserved protein (MtH895) from the thermophilic archeon Methanobacterium thermoautotrophicum. Our findings indicate that MtH895 contains a central four-stranded beta-sheet core surrounded by two helices on one side and a third on the other. It has an overall fold superficially similar to that of a glutaredoxin. However, detailed analysis of its three-dimensional structure along with molecular docking simulations of its interaction with T7 DNA polymerase (a thioredoxin-specific substrate) and comparisons with other known members of the thioredoxin/glutaredoxin family of proteins strongly suggest that MtH895 is more akin to a thioredoxin. Furthermore, measurement of the pK(a) values of its active site thiols along with direct measurements of the thioredoxin/glutaredoxin activity has confirmed that MtH895 is, indeed, a thioredoxin and exhibits no glutaredoxin activity. We have also identified a group of previously unknown proteins from several other archaebacteria that have significant (34-44%) sequence identity with MtH895. These proteins have unusual active site -CXXC- motifs not found in any known thioredoxin or glutaredoxin. On the basis of the results presented here, we predict that these small proteins are all members of a new class of truncated thioredoxins.
About this Structure
1ILO is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
Identification of a novel archaebacterial thioredoxin: determination of function through structure., Bhattacharyya S, Habibi-Nazhad B, Amegbey G, Slupsky CM, Yee A, Arrowsmith C, Wishart DS, Biochemistry. 2002 Apr 16;41(15):4760-70. PMID:11939770 Page seeded by OCA on Fri May 2 20:07:52 2008
Categories: Methanothermobacter thermautotrophicus | Single protein | Bhattacharyya, S. | Habibi-Nazhad, B. | NESG, Northeast Structural Genomics Consortium. | Slupsky, C M. | Sykes, B D. | Wishart, D S. | Beta-alpha-beta-alpha-beta-beta-alpha motif | Nesg | Northeast structural genomics consortium | Protein structure initiative | Psi | Structural genomic
