1in8
From Proteopedia
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'''THERMOTOGA MARITIMA RUVB T158V''' | '''THERMOTOGA MARITIMA RUVB T158V''' | ||
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[[Category: Tsuruta, H.]] | [[Category: Tsuruta, H.]] | ||
[[Category: Wetmur, J G.]] | [[Category: Wetmur, J G.]] | ||
| - | [[Category: | + | [[Category: Aaa+-class atpase]] |
| - | [[Category: | + | [[Category: Arginine finger]] |
| - | [[Category: | + | [[Category: Branch migration motor]] |
| - | [[Category: | + | [[Category: Dna translocase]] |
| - | [[Category: | + | [[Category: Holliday junction]] |
| - | [[Category: | + | [[Category: Sensor 1]] |
| - | [[Category: | + | [[Category: Sensor 2]] |
| - | [[Category: | + | [[Category: Walker some]] |
| - | [[Category: | + | [[Category: Walker b]] |
| - | [[Category: | + | [[Category: Winged-helix domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:10:57 2008'' | |
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Revision as of 17:10, 2 May 2008
THERMOTOGA MARITIMA RUVB T158V
Overview
The RuvB hexamer is the chemomechanical motor of the RuvAB complex that migrates Holliday junction branch-points in DNA recombination and the rescue of stalled DNA replication forks. The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant structures reveal that RuvB is an ATPase-associated with diverse cellular activities (AAA+-class ATPase) with a winged-helix DNA-binding domain. The RuvB-ADP complex structure and mutagenesis suggest how AAA+-class ATPases couple nucleotide binding and hydrolysis to interdomain conformational changes and asymmetry within the RuvB hexamer implied by the crystallographic packing and small-angle X-ray scattering in solution. ATP-driven domain motion is positioned to move double-stranded DNA through the hexamer and drive conformational changes between subunits by altering the complementary hydrophilic protein- protein interfaces. Structural and biochemical analysis of five motifs in the protein suggest that ATP binding is a strained conformation recognized both by sensors and the Walker motifs and that intersubunit activation occurs by an arginine finger motif reminiscent of the GTPase-activating proteins. Taken together, these results provide insights into how RuvB functions as a motor for branch migration of Holliday junctions.
About this Structure
1IN8 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of the RuvB Holliday junction branch migration motor., Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA, J Mol Biol. 2001 Aug 10;311(2):297-310. PMID:11478862 Page seeded by OCA on Fri May 2 20:10:57 2008
