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5jsz

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Revision as of 11:37, 17 June 2020

Folate ECF transporter: apo state

Structural highlights

5jsz is a 8 chain structure with sequence from "thermobacterium_bulgaricum"_orla-jensen_1919 "thermobacterium bulgaricum" orla-jensen 1919 and Lacda. This structure supersedes the now removed PDB entry 5d7t. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5d7t
Gene:	ecfA1, cbiO1, Ldb0424 (LACDA), ecfA2, cbiO2, Ldb0425 (LACDA), Ldb1625 (LACDA), ecfT, AT236_00396, CFL1_00754, SB57_06620 ("Thermobacterium bulgaricum" Orla-Jensen 1919)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[A0A061BSU4_LACDE] Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.[HAMAP-Rule:MF_01461] [ECFA1_LACDA] ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. [ECFA2_LACDA] ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.

Publication Abstract from PubMed

Energy-coupling factor (ECF) transporters mediate uptake of micronutrients in prokaryotes. The transporters consist of an S-component that binds the transported substrate and an ECF module (EcfAA'T) that binds and hydrolyses ATP. The mechanism of transport is poorly understood but presumably involves an unusual step in which the membrane-embedded S-component topples over to carry the substrate across the membrane. In many ECF transporters, the S-component dissociates from the ECF module after transport. Subsequently, substrate-bound S-components out-compete the empty proteins for re-binding to the ECF module in a new round of transport. Here we present crystal structures of the folate-specific transporter ECF-FolT from Lactobacillus delbrueckii. Interaction of the ECF module with FolT stabilizes the toppled state, and simultaneously destroys the high-affinity folate-binding site, allowing substrate release into the cytosol. We hypothesize that differences in the kinetics of toppling can explain how substrate-loaded FolT out-competes apo-FolT for association with the ECF module.

Structural insight in the toppling mechanism of an energy-coupling factor transporter.,Swier LJ, Guskov A, Slotboom DJ Nat Commun. 2016 Mar 30;7:11072. doi: 10.1038/ncomms11072. PMID:27026363^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Swier LJ, Guskov A, Slotboom DJ. Structural insight in the toppling mechanism of an energy-coupling factor transporter. Nat Commun. 2016 Mar 30;7:11072. doi: 10.1038/ncomms11072. PMID:27026363 doi:http://dx.doi.org/10.1038/ncomms11072

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5jsz"

@@ Line 1: / Line 1: @@
 ==Folate ECF transporter: apo state==
-<StructureSection load='5jsz' size='340' side='right' caption='[[5jsz]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+<StructureSection load='5jsz' size='340' side='right'caption='[[5jsz]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5jsz]] is a 8 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5d7t 5d7t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JSZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5jsz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermobacterium_bulgaricum"_orla-jensen_1919 "thermobacterium bulgaricum" orla-jensen 1919] and [http://en.wikipedia.org/wiki/Lacda Lacda]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5d7t 5d7t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JSZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JSZ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d7t|5d7t]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jsz OCA], [http://pdbe.org/5jsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jsz RCSB], [http://www.ebi.ac.uk/pdbsum/5jsz PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ecfA1, cbiO1, Ldb0424 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=390333 LACDA]), ecfA2, cbiO2, Ldb0425 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=390333 LACDA]), Ldb1625 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=390333 LACDA]), ecfT, AT236_00396, CFL1_00754, SB57_06620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1585 "Thermobacterium bulgaricum" Orla-Jensen 1919])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jsz OCA], [http://pdbe.org/5jsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jsz RCSB], [http://www.ebi.ac.uk/pdbsum/5jsz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jsz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Thermobacterium bulgaricum orla-jensen 1919]]
+[[Category: Lacda]]
+[[Category: Large Structures]]
 [[Category: Guskov, A]]
 [[Category: Slotboom, D J]]

5jsz

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Revision as of 11:37, 17 June 2020

Folate ECF transporter: apo state

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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