1io8

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[[Image:1io8.jpg|left|200px]]
[[Image:1io8.jpg|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1io8", creates the "Structure Box" on the page.
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{{STRUCTURE_1io8| PDB=1io8 | SCENE= }}
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|RELATEDENTRY=[[1io7|1IO7]], [[1io9|1IO9]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1io8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1io8 OCA], [http://www.ebi.ac.uk/pdbsum/1io8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1io8 RCSB]</span>
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'''THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES'''
'''THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES'''
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[[Category: Sligar, S G.]]
[[Category: Sligar, S G.]]
[[Category: Yamane, K.]]
[[Category: Yamane, K.]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: cytochromo p450]]
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[[Category: Cytochromo p450]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: thermophilic]]
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[[Category: Thermophilic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:12:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:22:25 2008''
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Revision as of 17:12, 2 May 2008

Image:1io8.jpg

Template:STRUCTURE 1io8

THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES


Overview

Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus solfataricus were determined at 1.5-2.0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the mutant crystal structure and differential scanning calorimetry.

About this Structure

1IO8 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties., Park SY, Yamane K, Adachi S, Shiro Y, Weiss KE, Maves SA, Sligar SG, J Inorg Biochem. 2002 Sep 20;91(4):491-501. PMID:12237217 Page seeded by OCA on Fri May 2 20:12:48 2008

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