1ioo
From Proteopedia
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'''CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE''' | '''CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IOO OCA]. | |
==Reference== | ==Reference== | ||
The 1.55 A resolution structure of Nicotiana alata S(F11)-RNase associated with gametophytic self-incompatibility., Ida K, Norioka S, Yamamoto M, Kumasaka T, Yamashita E, Newbigin E, Clarke AE, Sakiyama F, Sato M, J Mol Biol. 2001 Nov 16;314(1):103-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11724536 11724536] | The 1.55 A resolution structure of Nicotiana alata S(F11)-RNase associated with gametophytic self-incompatibility., Ida K, Norioka S, Yamamoto M, Kumasaka T, Yamashita E, Newbigin E, Clarke AE, Sakiyama F, Sato M, J Mol Biol. 2001 Nov 16;314(1):103-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11724536 11724536] | ||
| - | [[Category: Nicotiana alata]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Ida, K.]] | [[Category: Ida, K.]] | ||
[[Category: Kumasaka, T.]] | [[Category: Kumasaka, T.]] | ||
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[[Category: Yamamoto, M.]] | [[Category: Yamamoto, M.]] | ||
[[Category: Yamashita, E.]] | [[Category: Yamashita, E.]] | ||
| - | [[Category: | + | [[Category: Self-incompatibility ribonuclease]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:13:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:13, 2 May 2008
CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE
Overview
The crystal structure of Nicotiana alata (ornamental tobacco) S(F11)-RNase, an S-allelic glycoprotein associated with gametophytic self-incompatibility, was determined by X-ray diffraction at 1.55 A resolution. The protein has a tertiary structure typical of members of the RNase T(2) family as it consists of a variant of the (alpha+beta) fold and has eight helices and seven strands. A heptasaccharide moiety is also present, and amino acid residues that serve as the catalytic acid and base can be assigned to His32 and His91, respectively. Two "hypervariable" regions, known as HVa and HVb, are the proposed sites of S-allele discrimination during the self-incompatibility reaction, and in the S(F11)-RNase these are well separated from the active site. HVa and HVb are composed of a long, positively charged loop followed by a part of an alpha-helix and short, negatively charged alpha-helix, respectively. The S(F11)-RNase structure shows both regions are readily accessible to the solvent and hence could participate in the process of self/non-self discrimination between the S-RNase and an unknown pollen S-gene product(s) upon pollination.
About this Structure
Full crystallographic information is available from OCA.
Reference
The 1.55 A resolution structure of Nicotiana alata S(F11)-RNase associated with gametophytic self-incompatibility., Ida K, Norioka S, Yamamoto M, Kumasaka T, Yamashita E, Newbigin E, Clarke AE, Sakiyama F, Sato M, J Mol Biol. 2001 Nov 16;314(1):103-12. PMID:11724536 Page seeded by OCA on Fri May 2 20:13:49 2008
