1iov

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[[Image:1iov.gif|left|200px]]
[[Image:1iov.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1iov |SIZE=350|CAPTION= <scene name='initialview01'>1iov</scene>, resolution 2.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1iov", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=ADP:Adp+Binding+Site'>ADP</scene> and <scene name='pdbsite=LIG:Ligand+Binding+Site'>LIG</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POB:2-[(1-AMINO-ETHYL)-PHOSPHATE-PHOSPHINOYLOXY]-BUTYRIC+ACID'>POB</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/D-alanine--D-alanine_ligase D-alanine--D-alanine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.4 6.3.2.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1iov| PDB=1iov | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iov OCA], [http://www.ebi.ac.uk/pdbsum/1iov PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1iov RCSB]</span>
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}}
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'''COMPLEX OF D-ALA:D-ALA LIGASE WITH ADP AND A PHOSPHORYL PHOSPHONATE'''
'''COMPLEX OF D-ALA:D-ALA LIGASE WITH ADP AND A PHOSPHORYL PHOSPHONATE'''
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[[Category: Knox, J R.]]
[[Category: Knox, J R.]]
[[Category: Moews, P C.]]
[[Category: Moews, P C.]]
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[[Category: adp binding]]
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[[Category: Adp binding]]
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[[Category: cell wall]]
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[[Category: Cell wall]]
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[[Category: glycogen phosphorylase]]
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[[Category: Glycogen phosphorylase]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: peptidoglycan synthesis]]
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[[Category: Peptidoglycan synthesis]]
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[[Category: vancomycin]]
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[[Category: Vancomycin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:14:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:22:41 2008''
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Revision as of 17:14, 2 May 2008

Image:1iov.gif

Template:STRUCTURE 1iov

COMPLEX OF D-ALA:D-ALA LIGASE WITH ADP AND A PHOSPHORYL PHOSPHONATE


Overview

The crystallographic structure of the D-alanine:D-alanine ligase of the ddlB gene of Escherichia coli complexed with a D-Ala-D-alpha-hydroxybutyrate phosphonate and the structure of the Y216F mutant ligase complexed with a D-Ala-D-Ala phosphinate have been determined to 2.2 and 1.9 A resolution, respectively, and refined to R factors of 0.156 and 0.158. In each complex the inhibitor has reacted with ATP to produce ADP and a tight-binding phosphorylated transition state intermediate. Comparison of these two structures with the known crystal structure of the phosphinate intermediate of the wild-type ligase shows no major conformational changes, but B factors indicate differences in mobility of loops covering the binding site. The weaker inhibition of the Y216F mutant by both inhibitors is thought to be due in part to the loss of an interloop hydrogen bond. A similar mechanism may account for poor inhibition of VanA, the homologous D-Ala:D-lactate ligase produced by vancomycin-resistant enterococci.

About this Structure

1IOV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant., Fan C, Park IS, Walsh CT, Knox JR, Biochemistry. 1997 Mar 4;36(9):2531-8. PMID:9054558 Page seeded by OCA on Fri May 2 20:14:16 2008

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