1ipe
From Proteopedia
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'''TROPINONE REDUCTASE-II COMPLEXED WITH NADPH''' | '''TROPINONE REDUCTASE-II COMPLEXED WITH NADPH''' | ||
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[[Category: Yamada, Y.]] | [[Category: Yamada, Y.]] | ||
[[Category: Yamashita, A.]] | [[Category: Yamashita, A.]] | ||
| - | [[Category: | + | [[Category: Laue diffraction]] |
| - | [[Category: | + | [[Category: Oxidoreductase]] |
| - | [[Category: | + | [[Category: Reduction of tropinone to pseudotropine]] |
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Short-chain dehydrogenase]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Tropane alkaloid biosynthesis]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:15:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:15, 2 May 2008
TROPINONE REDUCTASE-II COMPLEXED WITH NADPH
Overview
To understand the catalytic mechanism of an enzyme, it is crucial to determine the crystallographic structures corresponding to the individual reaction steps. Here, we report two crystal structures of enzyme-substrate complexes prior to reaction initiation: tropinone reductase-II (TR-II)-NADPH and TR-II-NADPH-tropinone complexes, determined from the identical crystals. A combination of two kinetic crystallographic techniques, a continuous flow of the substrates and Laue diffraction measurements, enabled us to capture the transit structures prior to the reaction proceeding. A structure comparison of the enzyme-substrate complex elucidated in this study with the enzyme-product complex in our previous study indicates that one of the substrates, tropinone, is rotated relative to the product so as to make the spatial organization in the active site favorable for the reaction to proceed. Side chains of the residues in the active site also alter their conformations to keep the complementarity of the space for the substrate or the product and to assist the rotational movement.
About this Structure
1IPE is a Single protein structure of sequence from Datura stramonium. Full crystallographic information is available from OCA.
Reference
Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes., Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H, Biochemistry. 2003 May 20;42(19):5566-73. PMID:12741812 Page seeded by OCA on Fri May 2 20:15:17 2008
Categories: Datura stramonium | Single protein | Tropinone reductase II | Endo, M. | Higashi, T. | Kato, H. | Nakatsu, T. | Oda, J. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yamada, Y. | Yamashita, A. | Laue diffraction | Oxidoreductase | Reduction of tropinone to pseudotropine | Riken structural genomics/proteomics initiative | Rsgi | Short-chain dehydrogenase | Structural genomic | Tropane alkaloid biosynthesis
