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1ips

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[[Image:1ips.jpg|left|200px]]
[[Image:1ips.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ips |SIZE=350|CAPTION= <scene name='initialview01'>1ips</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1ips", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=SA:Active+Site+(Mn+Binding),+A+Chain'>SA</scene> and <scene name='pdbsite=SB:Active+Site+(Mn+Binding),+B+Chain'>SB</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= PCB C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 Emericella nidulans])
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-->
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|DOMAIN=
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{{STRUCTURE_1ips| PDB=1ips | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ips FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ips OCA], [http://www.ebi.ac.uk/pdbsum/1ips PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ips RCSB]</span>
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}}
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'''ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MANGANESE COMPLEX)'''
'''ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MANGANESE COMPLEX)'''
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[[Category: Roach, P L.]]
[[Category: Roach, P L.]]
[[Category: Schofield, C J.]]
[[Category: Schofield, C J.]]
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[[Category: antibiotic biosynthesis]]
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[[Category: Antibiotic biosynthesis]]
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[[Category: b-lactam antibiotic]]
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[[Category: B-lactam antibiotic]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: oxygenase]]
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[[Category: Oxygenase]]
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[[Category: penicillin biosynthesis]]
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[[Category: Penicillin biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:15:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:23:01 2008''
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Revision as of 17:15, 2 May 2008

Image:1ips.jpg

Template:STRUCTURE 1ips

ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (MANGANESE COMPLEX)


Overview

Penicillin antibiotics are all produced from fermentation-derived penicillins because their chemical synthesis is not commercially viable. The key step in penicillin biosynthesis, in which both the beta-lactam and thiazolidine rings of the nucleus are created, is mediated by isopenicillin N synthase (IPNS), which binds ferrous iron and uses dioxygen as a cosubstrate. In a unique enzymatic step, with no chemical precedent, IPNS catalyses the transfer of four hydrogen atoms from its tripeptide substrate to dioxygen forming, in a single reaction, the complete bicyclic nucleus of the penicillins. We now report the structure of IPNS complexed with manganese, which reveals the active site is unusually buried within a 'jelly-roll' motif and lined by hydrophobic residues, and suggest how this structure permits the process of penicillin formation. Sequence analyses indicate IPNS, 1-aminocyclopropane-1-carboxylic acid oxidase and many of the 2-oxo-acid-dependent oxygenases contain a conserved jelly-roll motif, forming a new structural family of enzymes.

About this Structure

1IPS is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.

Reference

Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes., Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE, Nature. 1995 Jun 22;375(6533):700-4. PMID:7791906 Page seeded by OCA on Fri May 2 20:15:54 2008

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