1is9

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[[Image:1is9.jpg|left|200px]]
[[Image:1is9.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1is9 |SIZE=350|CAPTION= <scene name='initialview01'>1is9</scene>, resolution 1.03&Aring;
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The line below this paragraph, containing "STRUCTURE_1is9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span>
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1is9| PDB=1is9 | SCENE= }}
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|RELATEDENTRY=[[1cem|1CEM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1is9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1is9 OCA], [http://www.ebi.ac.uk/pdbsum/1is9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1is9 RCSB]</span>
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}}
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'''Endoglucanase A from Clostridium thermocellum at atomic resolution'''
'''Endoglucanase A from Clostridium thermocellum at atomic resolution'''
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[[Category: Morris, R J.]]
[[Category: Morris, R J.]]
[[Category: Schmidt, A.]]
[[Category: Schmidt, A.]]
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[[Category: endoglucanase family 8]]
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[[Category: Endoglucanase family 8]]
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[[Category: hg derivative]]
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[[Category: Hg derivative]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:21:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:23:57 2008''
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Revision as of 17:21, 2 May 2008

Image:1is9.jpg

Template:STRUCTURE 1is9

Endoglucanase A from Clostridium thermocellum at atomic resolution


Overview

The structure of the endoglucanase A from Clostridium thermocellum CelA was re-solved by three-wavelength MAD. Experimental phases were obtained in the resolution range 25-1.0 A. Various structure-solution approaches were tested in order to quantify the contribution of each wavelength. Two-wavelength MAD phasing was sufficient to obtain excellent experimental phases. SAD at the remote wavelength also resulted in interpretable maps. The three-wavelength MAD electron-density map was of excellent quality: for parts of the structure, atom types and bond types could be easily assigned. Double bonds in peptide links and side chains could be located owing to their increased electron density indicating their pi character. Comparison with a previously determined structure of CelA at 1.65 A showed that, apart from a few additional multiple conformers and differently oriented side chains, major differences occur at the protein-solvent interface. A complete additional solvent shell could be observed and the inner shells have been completed. The high accuracy of the structure allowed unambiguous assignment of the protonation state for the active-site catalytic carboxylates.

About this Structure

1IS9 is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.

Reference

Advantages of high-resolution phasing: MAD to atomic resolution., Schmidt A, Gonzalez A, Morris RJ, Costabel M, Alzari PM, Lamzin VS, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1433-41. Epub 2002, Aug 23. PMID:12198299 Page seeded by OCA on Fri May 2 20:21:12 2008

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